MCB
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
MCB.02179-06v1
27/22/7802    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sinner, D.
Right arrow Articles by Wells, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sinner, D.
Right arrow Articles by Wells, J. M.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, November 2007, p. 7802-7815, Vol. 27, No. 22
0270-7306/07/$08.00+0     doi:10.1128/MCB.02179-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Sox17 and Sox4 Differentially Regulate ß-Catenin/T-Cell Factor Activity and Proliferation of Colon Carcinoma Cells{triangledown}

Débora Sinner,{dagger} Jennifer J. Kordich,{dagger} Jason R. Spence, Robert Opoka, Scott Rankin, Suh-Chin J. Lin, Diva Jonatan, Aaron M. Zorn,* and James M. Wells*

Division of Developmental Biology, Cincinnati Children's Hospital Research Foundation, 3333 Burnet Avenue, Cincinnati, Ohio 45229-3039

Received 21 November 2006/ Returned for modification 22 December 2006/ Accepted 6 September 2007

The canonical Wnt pathway is necessary for gut epithelial cell proliferation, and aberrant activation of this pathway causes intestinal neoplasia. We report a novel mechanism by which the Sox family of transcription factors regulate the canonical Wnt signaling pathway. We found that some Sox proteins antagonize while others enhance ß-catenin/T-cell factor (TCF) activity. Sox17, which is expressed in the normal gut epithelium but exhibits reduced expression in intestinal neoplasia, is antagonistic to Wnt signaling. When overexpressed in SW480 colon carcinoma cells, Sox17 represses ß-catenin/TCF activity in a dose-dependent manner and inhibits proliferation. Sox17 and Sox4 are expressed in mutually exclusive domains in normal and neoplastic gut tissues, and gain- and loss-of-function studies demonstrate that Sox4 enhances ß-catenin/TCF activity and the proliferation of SW480 cells. In addition to binding ß-catenin, both Sox17 and Sox4 physically interact with TCF/lymphoid enhancer factor (LEF) family members via their respective high-mobility-group box domains. Results from gain- and loss-of-function experiments suggest that the interaction of Sox proteins with ß-catenin and TCF/LEF proteins regulates the stability of ß-catenin and TCF/LEF. In particular, Sox17 promotes the degradation of both ß-catenin and TCF proteins via a noncanonical, glycogen synthase kinase 3ß-independent mechanism that can be blocked by proteasome inhibitors. In contrast, Sox4 may function to stabilize ß-catenin protein. These findings indicate that Sox proteins can act as both antagonists and agonists of ß-catenin/TCF activity, and this mechanism may regulate Wnt signaling responses in many developmental and disease contexts.


* Corresponding author. Mailing address: Division of Developmental Biology, Cincinnati Children's Hospital Research Foundation, 3333 Burnet Ave., Cincinnati, OH 45229-3039. Phone: (513) 636-8767. Fax: (513) 636-4317. E-mail for Aaron M. Zorn: aaron.zorn{at}cchmc.org. E-mail for James M. Wells: james.wells{at}cchmc.org

{triangledown} Published ahead of print on 17 September 2007.

{dagger} These authors contributed equally.


Molecular and Cellular Biology, November 2007, p. 7802-7815, Vol. 27, No. 22
0270-7306/07/$08.00+0     doi:10.1128/MCB.02179-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.




This article has been cited by other articles:




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. J. Virol. Eukaryot. Cell
Microbiol. Mol. Biol. Rev. Clin. Vaccine Immunol. All ASM Journals

Copyright © 2007 by the American Society for Microbiology. All rights reserved.