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 Previous Article

Molecular and Cellular Biology, November 2007, p. 8015-8026, Vol. 27, No. 22
0270-7306/07/$08.00+0     doi:10.1128/MCB.01102-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Hmo1 Is Required for TOR-Dependent Regulation of Ribosomal Protein Gene Transcription{triangledown} ,{dagger}

Axel B. Berger,1 Laurence Decourty,2 Gwenaël Badis,2 Ulf Nehrbass,1* Alain Jacquier,2 and Olivier Gadal1,3*

Unité de Biologie Cellulaire du Noyau, CNRS URA 2582,1 Unité de Génétique des Interactions Macromoléculaires, CNRS URA 2171, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris cedex 15, France,2 Organisation et Dynamique Nucléaire, LBME-CNRS, Université de Toulouse, 118 route de Narbonne, 31000 Toulouse, France3

Received 21 June 2007/ Returned for modification 26 July 2007/ Accepted 3 September 2007

Ribosome biogenesis requires equimolar amounts of four rRNAs and all 79 ribosomal proteins (RP). Coordinated regulation of rRNA and RP synthesis by eukaryotic RNA polymerases (Pol) I, III, and II is a key requirement for growth control. Using a novel global genetic approach, we showed that the absence of Hmo1 becomes lethal when combined with mutations of components of either the RNA Pol II or Pol I transcription machineries, of specific RP, or of the TOR pathway. Hmo1 directly interacts with both the region transcribed by Pol I and a subset of RP gene promoters. Down-regulation of Hmo1 expression affects RP gene expression. Upon TORC1 inhibition, Hmo1 dissociates from ribosomal DNA (rDNA) and some RP gene promoters simultaneously. Finally, in the absence of Hmo1, TOR-dependent repression of RP genes is alleviated. Therefore, we show here that Saccharomyces cerevisiae Hmo1 is directly involved in coordinating rDNA transcription by Pol I and RP gene expression by Pol II under the control of the TOR pathway.

* Corresponding author. Mailing address for Ulf Nehrbass: Unité de Biologie Cellulaire du Noyau, CNRS URA 2582, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris cedex 15, France. Phone: 33 (0)1 40 61 39 13. Fax: 33 (0)1 40 61 39 18. E-mail: Nehrbass{at}pasteur.fr. Mailing address for Olivier Gadal: Organisation et Dynamique Nucléaire, LBME-CNRS, Université de Toulouse, 118 route de Narbonne, 31000 Toulouse, France. Phone: 33 (0)561 33 59 39. Fax: 33 (0)561 33 58 86. E-mail: Olivier.Gadal{at}ibcg.biotoul.fr

{triangledown} Published ahead of print on 17 September 2007.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, November 2007, p. 8015-8026, Vol. 27, No. 22
0270-7306/07/$08.00+0     doi:10.1128/MCB.01102-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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