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Molecular and Cellular Biology, December 2007, p. 8306-8317, Vol. 27, No. 23
0270-7306/07/$08.00+0     doi:10.1128/MCB.01351-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Domain Architecture of the Catalytic Subunit in the ISW2-Nucleosome Complex{triangledown}

Weiwei Dang{dagger} and Blaine Bartholomew*

Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, 1245 Lincoln Dr., Carbondale, Illinois 62901-4413

Received 27 July 2007/ Returned for modification 17 August 2007/ Accepted 17 September 2007

ATP-dependent chromatin remodeling has an important role in the regulation of cellular differentiation and development. For the first time, a topological view of one of these complexes has been revealed, by mapping the interactions of the catalytic subunit Isw2 with nucleosomal and extranucleosomal DNA in the complex with all four subunits of ISW2 bound to nucleosomes. Different domains of Isw2 were shown to interact with the nucleosome near the dyad axis, another near the entry site of the nucleosome, and another with extranucleosomal DNA. The conserved DEXD or ATPase domain was found to contact the superhelical location 2 (SHL2) of the nucleosome, providing a direct physical connection of ATP hydrolysis with this region of nucleosomes. The C terminus of Isw2, comprising the SLIDE (SANT-like domain) and HAND domains, was found to be associated with extranucleosomal DNA and the entry site of nucleosomes. It is thus proposed that the C-terminal domains of Isw2 are involved in anchoring the complex to nucleosomes through their interactions with linker DNA and that they facilitate the movement of DNA along the surface of nucleosomes.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, 1245 Lincoln Dr., Neckers Bldg., Room 229, Carbondale, IL 62901-4413. Phone: (618) 453-6437. Fax: (618) 453-6440. E-mail: bbartholomew{at}siumed.edu

{triangledown} Published ahead of print on 1 October 2007.

{dagger} Present address: The Wistar Institute, 3601 Spruce St., Philadelphia, PA 19104.

Molecular and Cellular Biology, December 2007, p. 8306-8317, Vol. 27, No. 23
0270-7306/07/$08.00+0     doi:10.1128/MCB.01351-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pinskaya, M., Nair, A., Clynes, D., Morillon, A., Mellor, J. (2009). Nucleosome Remodeling and Transcriptional Repression Are Distinct Functions of Isw1 in Saccharomyces cerevisiae. Mol. Cell. Biol. 29: 2419-2430 [Abstract] [Full Text]  


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