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Molecular and Cellular Biology, December 2007, p. 8401-8408, Vol. 27, No. 23
0270-7306/07/$08.00+0     doi:10.1128/MCB.01478-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Complex Formation of Yeast Rev1 with DNA Polymerase {eta}{triangledown}

Narottam Acharya,1 Lajos Haracska,2 Satya Prakash,1 and Louise Prakash1*

Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555-1061,1 Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary2

Received 15 August 2007/ Returned for modification 6 September 2007/ Accepted 11 September 2007

In Saccharomyces cerevisiae, Rev1 functions in translesion DNA synthesis (TLS) together with polymerase {zeta} (Pol{zeta}), comprised of the Rev3 catalytic and Rev7 accessory subunits. Rev1 plays an indispensable structural role in promoting Pol{zeta} function, and deletion of the Rev1-C terminal region that is involved in physical interactions with Rev3 inactivates Pol{zeta} function in TLS. In humans, however, Rev1 has been shown to physically interact with the Y-family polymerases Pol{eta}, Pol{iota}, and Pol{kappa}, and the Rev1 C terminus mediates these interactions. Since all the available genetic and biochemical evidence in yeast support the requirement of Rev1 as a structural element for Pol{zeta} and not for Pol{eta}, these observations have raised the possibility that in its structural role, Rev1 has diverged between yeast and humans. Here we show that although in yeast a stable Rev1-Pol{eta} complex can be formed, this complex formation involves the polymerase-associated domain of Rev1 and not the Rev1 C terminus as in humans. We also found that the DNA synthesis activity of Rev1 is enhanced in this complex. We discuss the implications of these and other observations for the possible divergence of Rev1's structural role between yeast and humans.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, University of Texas Medical Branch at Galveston, 6.104 Blocker Medical Research Building, 301 University Blvd., Galveston, TX 77555-1061. Phone: (409) 747-8601. Fax: (409) 747-8608. E-mail: l.prakash{at}utmb.edu

{triangledown} Published ahead of print on 17 September 2007.

Molecular and Cellular Biology, December 2007, p. 8401-8408, Vol. 27, No. 23
0270-7306/07/$08.00+0     doi:10.1128/MCB.01478-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Acharya, N., Johnson, R. E., Pages, V., Prakash, L., Prakash, S. (2009). Yeast Rev1 protein promotes complex formation of DNA polymerase {zeta} with Pol32 subunit of DNA polymerase {delta}. Proc. Natl. Acad. Sci. USA 106: 9631-9636 [Abstract] [Full Text]  
  • Waters, L. S., Minesinger, B. K., Wiltrout, M. E., D'Souza, S., Woodruff, R. V., Walker, G. C. (2009). Eukaryotic Translesion Polymerases and Their Roles and Regulation in DNA Damage Tolerance. Microbiol. Mol. Biol. Rev. 73: 134-154 [Abstract] [Full Text]  


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