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Molecular and Cellular Biology, December 2007, p. 8492-8501, Vol. 27, No. 24
0270-7306/07/$08.00+0     doi:10.1128/MCB.01173-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Protein Interactions Involved in tRNA Gene-Specific Integration of Dictyostelium discoideum Non-Long Terminal Repeat Retrotransposon TRE5-A{triangledown}

Thanh Chung,1 Oliver Siol,2 Theodor Dingermann,1,3 and Thomas Winckler2*

Institut für Pharmazeutische Biologie, Universität Frankfurt am Main, Frankfurt am Main, Germany,1 Lehrstuhl für Pharmazeutische Biologie, Universität Jena, Jena, Germany,2 Zentrum für Arzneimittelforschung, Entwicklung und Sicherheit, Frankfurt am Main, Germany3

Received 2 July 2007/ Returned for modification 25 September 2007/ Accepted 28 September 2007

Mobile genetic elements that reside in gene-dense genomes face the problem of avoiding devastating insertional mutagenesis of genes in their host cell genomes. To meet this challenge, some Saccharomyces cerevisiae long terminal repeat (LTR) retrotransposons have evolved targeted integration at safe sites in the immediate vicinity of tRNA genes. Integration of yeast Ty3 is mediated by interactions of retrotransposon protein with the tRNA gene-specific transcription factor IIIB (TFIIIB). In the genome of the social amoeba Dictyostelium discoideum, the non-LTR retrotransposon TRE5-A integrates ~48 bp upstream of tRNA genes, yet little is known about how the retrotransposon identifies integration sites. Here, we show direct protein interactions of the TRE5-A ORF1 protein with subunits of TFIIIB, suggesting that ORF1p is a component of the TRE5-A preintegration complex that determines integration sites. Our results demonstrate that evolution has put forth similar solutions to prevent damage of diverse, compact genomes by different classes of mobile elements.

* Corresponding author. Mailing address: Lehrstuhl für Pharmazeutische Biologie, Universität Jena, Semmelweisstrasse 10, D-07743 Jena, Germany. Phone: 49-3641-949841. Fax: 49-3641-949842. E-mail: t.winckler{at}uni-jena.de

{triangledown} Published ahead of print on 8 October 2007.

Molecular and Cellular Biology, December 2007, p. 8492-8501, Vol. 27, No. 24
0270-7306/07/$08.00+0     doi:10.1128/MCB.01173-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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