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Molecular and Cellular Biology, December 2007, p. 8739-8747, Vol. 27, No. 24
0270-7306/07/$08.00+0     doi:10.1128/MCB.01304-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Drosophila Blimp-1 Is a Transient Transcriptional Repressor That Controls Timing of the Ecdysone-Induced Developmental Pathway{triangledown}

Yasuo Agawa,1,{dagger},{ddagger} Moustafa Sarhan,2,{dagger} Yuji Kageyama,1,§ Kazutaka Akagi,2 Masayoshi Takai,2 Kazuya Hashiyama,1,§ Tadashi Wada,3,4 Hiroshi Handa,3 Akihiro Iwamatsu,5 Susumu Hirose,1 and Hitoshi Ueda2*

Department of Developmental Genetics, National Institute of Genetics and Department of Genetics, The Graduate University for Advanced Studies, 1111 Yata, Mishima, Shizuoka 411-8540, Japan,1 The Graduate School of Natural Science and Technology and Department of Biology, Faculty of Science, Okayama University, 3-1-1 Tsushima-naka, Okayama 700-8530, Japan,2 Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8501, Japan,3 Integrated Research Institute, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8501, Japan,4 Protein Research Network, Inc., Kanagawa-ku, Yokohama 236-0004, Japan5

Received 20 July 2007/ Returned for modification 28 August 2007/ Accepted 27 September 2007

Regulatory mechanisms controlling the timing of developmental events are crucial for proper development to occur. ftz-f1 is expressed in a temporally regulated manner following pulses of ecdysteroid and this precise expression is necessary for the development of Drosophila melanogaster. To understand how insect hormone ecdysteroids regulate the timing of FTZ-F1 expression, we purified a DNA binding regulator of ftz-f1. Mass spectroscopy analysis revealed this protein to be a fly homolog of mammalian B lymphocyte-induced maturation protein 1 (Blimp-1). Drosophila Blimp-1 (dBlimp-1) is induced directly by 20-hydroxyecdysone, and its product exists during high-ecdysteroid periods and turns over rapidly. Forced expression of dBlimp-1 and RNA interference analysis indicate that dBlimp-1 acts as a repressor and controls the timing of FTZ-F1 expression. Furthermore, its prolonged expression results in delay of pupation timing. These results suggest that the transient transcriptional repressor dBlimp-1 is important for determining developmental timing in the ecdysone-induced pathway.

* Corresponding author. Mailing address: The Graduate School of Natural Science and Technology and Department of Biology, Faculty of Science, Okayama University, 3-1-1 Tsushima-naka, Okayama 700-8530, Japan. Phone: 81-86-251-7869. Fax: 81-86-251-7876. E-mail: hueda{at}cc.okayama-u.ac.jp

{triangledown} Published ahead of print on 8 October 2007.

{dagger} Yasuo Agawa and Moustafa Sarhan contributed equally to this work.

{ddagger} Present address: Drosophila Genetic Resource Center, Kyoto Institute of Technology, Sagaippongi-cho, Ukyou-ku, Kyoto 616-8354, Japan.

§ Present address: Okazaki Institute for Integrated Bioscience, National Institutes of Natural Sciences, 5-1 Myodaiji-Higashiyama, Okazaki, Aichi 444-8787, Japan.

Molecular and Cellular Biology, December 2007, p. 8739-8747, Vol. 27, No. 24
0270-7306/07/$08.00+0     doi:10.1128/MCB.01304-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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