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Molecular and Cellular Biology, February 2007, p. 1495-1504, Vol. 27, No. 4
0270-7306/07/$08.00+0     doi:10.1128/MCB.01764-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

p18Ink4c, but Not p27Kip1, Collaborates with Men1 To Suppress Neuroendocrine Organ Tumors{triangledown}

Feng Bai,{dagger} Xin-Hai Pei,{dagger} Toru Nishikawa,{ddagger} Matthew D. Smith, and Yue Xiong*

Lineberger Comprehensive Cancer Center, Department of Biochemistry and Biophysics, Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7295

Received 18 September 2006/ Returned for modification 22 October 2006/ Accepted 25 November 2006

Mutant mice lacking both cyclin-dependent kinase (CDK) inhibitors p18Ink4c and p27Kip1 develop a tumor spectrum reminiscent of human multiple endocrine neoplasia (MEN) syndromes. To determine how p18 and p27 genetically interact with Men1, the tumor suppressor gene mutated in familial MEN1, we characterized p18-Men1 and p27-Men1 double mutant mice. Compared with their corresponding single mutant littermates, the p18–/–; Men1+/– mice develop tumors at an accelerated rate and with an increased incidence in the pituitary, thyroid, parathyroid, and pancreas. In the pituitary and pancreatic islets, phosphorylation of the retinoblastoma (Rb) protein at both CDK2 and CDK4/6 sites was increased in p18–/– and Men1+/– cells and was further increased in p18–/–; Men1+/– cells. The remaining wild-type Men1 allele was lost in most tumors from Men1+/– mice but was retained in most tumors from p18–/–; Men1+/– mice. Combined mutations of p27–/– and Men1+/–, in contrast, did not exhibit noticeable synergistic stimulation of Rb kinase activity, cell proliferation, and tumor growth. These results demonstrate that functional collaboration exists between p18 and Men1 and suggest that menin may regulate additional factor(s) that interact with p18 and p27 differently.

* Corresponding author. Mailing address: Lineberger Comprehensive Cancer Center, Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7295. Phone: (919) 962-2142. Fax: (919) 966-8799. E-mail: yxiong{at}email.unc.edu.

{triangledown} Published ahead of print on 4 December 2006.

{dagger} F.B. and X.-H.P. contributed equally to this work.

{ddagger} Present address: Division of Breast and Endocrine Surgery, St. Marianna University School of Medicine, Kawasaki, Japan.

Molecular and Cellular Biology, February 2007, p. 1495-1504, Vol. 27, No. 4
0270-7306/07/$08.00+0     doi:10.1128/MCB.01764-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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