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Molecular and Cellular Biology, April 2007, p. 2791-2799, Vol. 27, No. 8
0270-7306/07/$08.00+0     doi:10.1128/MCB.01445-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

A+U-Rich Instability Elements Differentially Activate 5'-3' and 3'-5' mRNA Decay{triangledown}

Elizabeth L. Murray and Daniel R. Schoenberg*

Department of Molecular and Cellular Biochemistry, RNA Group, and Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio 43210-1218

Received 4 August 2006/ Returned for modification 1 September 2006/ Accepted 5 February 2007

The A+U-rich elements (or AREs) are cis-acting sequences that activate rapid mRNA decay, yet the overall polarity of this process is unknown. The current study describes an unbiased approach to this using the Invader RNA assay (Third Wave Technologies, Inc.) to quantify the decay of each of the three exons of human ß-globin mRNA without added instability elements or with the AREs from c-fos or granulocyte-macrophage colony-stimulating factor (GM-CSF) mRNA in the 3' untranslated region. Each of these genes under tetracycline operator control was stably transfected into cells, and ß-globin mRNA was quantified with exon-specific probes following transcription termination. There was little overall evidence for polarity in stable mRNA decay. Adding the c-fos ARE activated rapid and simultaneous decay from both ends of the mRNA. In contrast, the GM-CSF ARE activated decay primarily from the mRNA 5' end. These data were supported by reciprocal RNA interference knockdowns, and we present evidence that the 5'-3' and 3'-5' decay pathways are functionally linked.

* Corresponding author. Mailing address: Department of Molecular and Cellular Biochemistry, The Ohio State University, 1645 Neil Ave., Columbus, OH 43210-1218. Phone: (614) 688-3012. Fax: (614) 292-4118. E-mail: schoenberg.3{at}osu.edu

{triangledown} Published ahead of print on 12 February 2007.

Molecular and Cellular Biology, April 2007, p. 2791-2799, Vol. 27, No. 8
0270-7306/07/$08.00+0     doi:10.1128/MCB.01445-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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