MCB
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
MCB.02159-06v1
27/8/2821    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kopcewicz, K. A.
Right arrow Articles by Reines, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kopcewicz, K. A.
Right arrow Articles by Reines, D.
Molecular and Cellular Biology, April 2007, p. 2821-2829, Vol. 27, No. 8
0270-7306/07/$08.00+0     doi:10.1128/MCB.02159-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Metabolic Regulation of IMD2 Transcription and an Unusual DNA Element That Generates Short Transcripts{triangledown}

Katarzyna A. Kopcewicz, Thomas W. O'Rourke, and Daniel Reines*

Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322

Received 17 November 2006/ Returned for modification 19 December 2006/ Accepted 31 January 2007

Transcriptional regulation of IMD2 in yeast (Saccharomyces cerevisiae) is governed by the concentration of intracellular guanine nucleotide pools. The mechanism by which pool size is measured and transduced to the transcriptional apparatus is unknown. Here we show that DNA sequences surrounding the IMD2 initiation site constitute a repressive element (RE) involved in guanine regulation that contains a novel transcription-blocking activity. When this regulatory region is placed downstream of a heterologous promoter, short poly(A)+ transcripts are generated. The element is orientation dependent, and sequences within the normally transcribed and nontranscribed regions of the element are required for its activity. The promoter-proximal short RNAs are unstable and serve as substrates for the nuclear exosome. These findings support a model in which intergenic short transcripts emanating from upstream of the IMD2 promoter are terminated by a polyadenylation/terminator-like signal embedded within the IMD2 transcription start site.

* Corresponding author. Mailing address: Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Rd., Atlanta, GA 30322. Phone: (404) 727-3361. Fax: (404) 727-2538. E-mail: dreines{at}emory.edu

{triangledown} Published ahead of print on 12 February 2007.

Molecular and Cellular Biology, April 2007, p. 2821-2829, Vol. 27, No. 8
0270-7306/07/$08.00+0     doi:10.1128/MCB.02159-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. J. Virol. Eukaryot. Cell
Microbiol. Mol. Biol. Rev. Clin. Vaccine Immunol. All ASM Journals

Copyright © 2007 by the American Society for Microbiology. All rights reserved.