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Molecular and Cellular Biology, April 2007, p. 2980-2986, Vol. 27, No. 8
0270-7306/07/$08.00+0     doi:10.1128/MCB.02337-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

ß-Globin Intergenic Transcription and Histone Acetylation Dependent on an Enhancer

AeRi Kim,^1,2 Hui Zhao,^1, Ina Ifrim,^1, and Ann Dean^1*

Laboratory of Cellular and Developmental Biology, NIDDK, NIH, Bethesda, Maryland 20892,¹ Department of Molecular Biology, College of Natural Sciences, Pusan National University, Pusan 609-735, South Korea²

Received 15 December 2006/ Returned for modification 16 January 2007/ Accepted 24 January 2007

Histone acetyltransferases are associated with the elongating RNA polymerase II (Pol II) complex, supporting the idea that histone acetylation and transcription are intertwined mechanistically in gene coding sequences. Here, we studied the establishment and function of histone acetylation and transcription in noncoding sequences by using a model locus linking the ß-globin HS2 enhancer and the embryonic -globin gene in chromatin. An intact HS2 enhancer that recruits RNA Pol II is required for intergenic transcription and histone H3 acetylation and K4 methylation between the enhancer and target gene. RNA Pol II recruitment to the target gene TATA box is not required for the intergenic transcription or intergenic histone modifications, strongly implying that they are properties conferred by the enhancer. However, Pol II recruitment at HS2, intergenic transcription, and intergenic histone modification are not sufficient for transcription or modification of the target gene: these changes require initiation at the TATA box of the gene. The results suggest that intergenic and genic transcription complexes are independent and possibly differ from one another.

Published ahead of print on 5 February 2007.

Present address: Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Seattle, WA 98109.

Present address: Laboratory of Immunology, NIAID, NIH, 10 Center Drive, MSC 1892, Bethesda, MD 20892.

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