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Molecular and Cellular Biology, April 2007, p. 3087-3097, Vol. 27, No. 8
0270-7306/07/$08.00+0     doi:10.1128/MCB.01876-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

The SR Family Proteins B52 and dASF/SF2 Modulate Development of the Drosophila Visual System by Regulating Specific RNA Targets{triangledown} ,{dagger}

Mathieu Gabut,1,{ddagger} Jérôme Dejardin,2,§ Jamal Tazi,1* and Johann Soret1*

CNRS, UMR 5535, Institut de Génétique Moléculaire de Montpellier, Montpellier F-34293, France,1 CNRS, UPR 1142, Institut de Génétique Humaine, CNRS, Montpellier F-34293, France2

Received 5 October 2006/ Returned for modification 9 November 2006/ Accepted 18 January 2007

Deciphering the role of alternative splicing in developmental processes relies on the identification of key genes whose expression is controlled by splicing regulators throughout the growth of a whole organism. Modulating the expression levels of five SR proteins in the developing eye of Drosophila melanogaster revealed that these splicing factors induce various phenotypic alterations in eye organogenesis and also affect viability. Although the SR proteins dASF/SF2 and B52 caused defects in ommatidia structure, only B52 impaired normal axonal projections of photoreceptors and neurogenesis in visual ganglia. Microarray analyses revealed that many transcripts involved in brain organogenesis have altered splicing profiles upon both loss and gain of B52 function. Conversely, a large proportion of transcripts regulated by dASF/SF2 are involved in eye development. These differential and specific effects of SR proteins indicate that they function to confer accuracy to developmental gene expression programs by facilitating the cell lineage decisions that underline the generation of tissue identities.

* Corresponding author. Mailing address: CNRS, UMR 5535, Institut de Génétique Moléculaire de Montpellier, Montpellier F-34293, France. Phone: 33 (0) 4 67 61 36 85. Fax: 33 (0) 4 67 04 02 31. E-mail for Johann Soret: johann.soret{at}igmm.cnrs.fr. E-mail for Jamal Tazi: jamal.tazi{at}igmm.cnrs.fr

{triangledown} Published ahead of print on 5 February 2007.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

{ddagger} Present address: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Canada M5S 3E1.

§ Present address: Harvard Medical School, Department of Genetics, and Massachusetts General Hospital, Department of Molecular Biology, Boston, MA 02114.

Molecular and Cellular Biology, April 2007, p. 3087-3097, Vol. 27, No. 8
0270-7306/07/$08.00+0     doi:10.1128/MCB.01876-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Marcucci, R., Romano, M., Feiguin, F., O'Connell, M. A., Baralle, F. E. (2009). Dissecting the splicing mechanism of the Drosophila editing enzyme; dADAR. Nucleic Acids Res 37: 1663-1671 [Abstract] [Full Text]  


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