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Molecular and Cellular Biology, January 2008, p. 20-29, Vol. 28, No. 1
0270-7306/08/$08.00+0     doi:10.1128/MCB.01122-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Telomere Loops and Homologous Recombination-Dependent Telomeric Circles in a Kluyveromyces lactis Telomere Mutant Strain{triangledown}

Anthony J. Cesare ,1,{dagger},{ddagger} Cindy Groff-Vindman,2,{ddagger} Sarah A. Compton,1 Michael J. McEachern,2 and Jack D. Griffith1*

Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599,1 Department of Genetics, University of Georgia, Athens, Georgia 306022

Received 24 June 2007/ Returned for modification 20 July 2007/ Accepted 10 October 2007

The Kluyveromyces lactis ter1-16T strain contains mutant telomeres that are poorly bound by Rap1, resulting in a telomere-uncapping phenotype and significant elongation of the telomeric DNA. The elongated telomeres of ter1-16T allowed the isolation and examination of native yeast telomeric DNA by electron microscopy. In the telomeric DNA isolated from ter1-16T, looped molecules were observed with the physical characteristics of telomere loops (t-loops) previously described in mammalian and plant cells. ter1-16T cells were also found to contain free circular telomeric DNA molecules (t-circles) ranging up to the size of an entire telomere. When the ter1-16T uncapping phenotype was repressed by overexpression of RAP1 or recombination was inhibited by deletion of rad52, the isolated telomeric DNA contained significantly fewer t-loops and t-circles. These results suggest that disruption of Rap1 results in elevated recombination at telomeres, leading to increased strand invasion of the 3' overhang within t-loop junctions and resolution of the t-loop junctions into free t-circles.

* Corresonding author. Mailing address: Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599. Phone: (919) 966-8563. Fax: (919) 966-3015. E-mail: jdg{at}med.unc.edu

{triangledown} Published ahead of print on 29 October 2007.

{dagger} Present address: Children's Medical Research Institute, Westmead, New South Wales 2150, Australia.

{ddagger} A.J.C. and C.G.-V. shared first authorship.

Molecular and Cellular Biology, January 2008, p. 20-29, Vol. 28, No. 1
0270-7306/08/$08.00+0     doi:10.1128/MCB.01122-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Basenko, E. Y., Cesare, A. J., Iyer, S., Griffith, J. D., McEachern, M. J. (2009). Telomeric circles are abundant in the stn1-M1 mutant that maintains its telomeres through recombination. Nucleic Acids Res 0: gkp814v1-gkp814 [Abstract] [Full Text]  
  • Grandin, N., Charbonneau, M. (2009). Telomerase- and Rad52-Independent Immortalization of Budding Yeast by an Inherited-Long-Telomere Pathway of Telomeric Repeat Amplification. Mol. Cell. Biol. 29: 965-985 [Abstract] [Full Text]  


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