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The PIAS-Like Protein Zimp10 Is Essential for Embryonic Viability and Proper Vascular Development

Departments of Urology,¹ Pathology,² Genetics, Stanford University School of Medicine, Stanford, California 94305-5328,³ Moores UCSD Cancer Center, University of California, San Diego, La Jolla, California 92093-0819,⁴ Department of Comparative Medicine, University of California, Davis, Davis, California 95616⁵

Received 2 May 2007/ Returned for modification 11 June 2007/ Accepted 12 October 2007

Members of the PIAS (for protein inhibitor of activated STAT) family play critical roles in modulating the activity of a variety of transcriptional regulators. Zimp10, a novel PIAS-like protein, is a transcriptional coregulator and may be involved in the modification of chromatin through interactions with the SWI/SNF chromatin-remodeling complexes. Here, we investigate the biological role of Zimp10 in zimp10-deficient mice. Homozygosity for the Zimp10-targeted allele resulted in developmental arrest at approximately embryonic day 10.5. Analysis of knockout embryos revealed severe defects in the reorganization of the yolk sac vascular plexus. No significant abnormality in hematopoietic potential was observed in zimp10 null mice. Microarray and quantified reverse transcription-PCR analyses showed that the expression of the Fos family member Fra-1, which is involved in extraembryonic vascular development, was reduced in yolk sac tissues of zimp10 null embryos. Using fra-1 promoter/reporter constructs, we further demonstrate the regulatory role of Zimp10 on the transcription of Fra-1. This study provides evidence to demonstrate a crucial role for Zimp10 in vasculogenesis.

Published ahead of print on 29 October 2007.