MCB
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
MCB.02227-07v1
28/10/3477    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Onishi, Y.
Right arrow Articles by Ishida, N.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Onishi, Y.
Right arrow Articles by Ishida, N.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, May 2008, p. 3477-3488, Vol. 28, No. 10
0270-7306/08/$08.00+0     doi:10.1128/MCB.02227-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Rhythmic SAF-A Binding Underlies Circadian Transcription of the Bmal1 Gene{triangledown}

Yoshiaki Onishi,1* Syuji Hanai,1 Tomoya Ohno,1,2 Yasuhiro Hara,1,2 and Norio Ishida1,2*

Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba 305-8566, Japan,1 Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8576, Japan2

Received 16 December 2007/ Returned for modification 6 February 2008/ Accepted 29 February 2008

Although Bmal1 is a key component of the mammalian clock system, little is understood about the actual mechanism of circadian Bmal1 gene transcription, particularly at the chromatin level. Here we discovered a unique chromatin structure within the Bmal1 promoter. The RORE region, which is a critical cis element for the circadian regulation of the Bmal1 gene, is comprised of GC-rich open chromatin. The 3'-flanking region of the promoter inhibited rhythmic transcription in the reporter gene assay in vitro even in the presence of ROR{alpha} and REV-ERB{alpha}. We also found that the nuclear matrix protein SAF-A binds to the 3'-flanking region with circadian timing, which was correlated with Bmal1 expression by footprinting in vivo. These results suggest that the unique chromatin structure containing SAF-A is required for the circadian transcriptional regulation of the Bmal1 gene in cells.

* Corresponding author. Mailing address: Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. Phone: 81-29-861-6053. Fax: 81-29-861-9499. E-mail for Yoshiaki Onishi: y-onishi{at}aist.go.jp. E-mail for Norio Ishida: n.ishida{at}aist.go.jp

{triangledown} Published ahead of print on 10 March 2008.

Molecular and Cellular Biology, May 2008, p. 3477-3488, Vol. 28, No. 10
0270-7306/08/$08.00+0     doi:10.1128/MCB.02227-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. J. Virol. Eukaryot. Cell
Microbiol. Mol. Biol. Rev. Clin. Vaccine Immunol. All ASM Journals

Copyright © 2008 by the American Society for Microbiology. All rights reserved.