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Molecular and Cellular Biology, June 2008, p. 4188-4195, Vol. 28, No. 12
0270-7306/08/$08.00+0     doi:10.1128/MCB.00229-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Functional Interaction between the Fab-7 and Fab-8 Boundaries and the Upstream Promoter Region in the Drosophila Abd-B Gene{triangledown}

Olga Kyrchanova, Stepan Toshchakov, Yulia Podstreshnaya, Alexander Parshikov, and Pavel Georgiev*

Department of the Control of Genetic Processes, Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilov St., Moscow 119334, Russia

Received 12 February 2008/ Accepted 5 April 2008

Boundary elements have been found in the regulatory region of the Drosophila melanogaster Abdominal-B (Abd-B) gene, which is subdivided into a series of iab domains. The best-studied Fab-7 and Fab-8 boundaries flank the iab-7 enhancer and isolate it from the four promoters regulating Abd-B expression. Recently binding sites for the Drosophila homolog of the vertebrate insulator protein CTCF (dCTCF) were identified in the Fab-8 boundary and upstream of Abd-B promoter A, with no binding of CTCF to the Fab-7 boundary being detected either in vivo or in vitro. Taking into account the inability of the yeast GAL4 activator to stimulate the white promoter when its binding sites are separated by a 5-kb yellow gene, we have tested the functional interactions between the Fab-7 and Fab-8 boundaries and between these boundaries and the upstream promoter A region containing a dCTCF binding site. It has been found that dCTCF binding sites are essential for pairing between two Fab-8 insulators. However, a strong functional interaction between the Fab-7 and Fab-8 boundaries suggests that additional, as yet unidentified proteins are involved in long-distance interactions between them. We have also shown that Fab-7 and Fab-8 boundaries effectively interact with the upstream region of the Abd-B promoter.

* Corresponding author. Mailing address: Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilov St., Moscow 119334, Russia. Phone: 7-495-1359734. Fax: 7-495-1354105. E-mail: georgiev_p{at}mail.ru

{triangledown} Published ahead of print on 21 April 2008.

Molecular and Cellular Biology, June 2008, p. 4188-4195, Vol. 28, No. 12
0270-7306/08/$08.00+0     doi:10.1128/MCB.00229-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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