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Molecular and Cellular Biology, August 2008, p. 4719-4733, Vol. 28, No. 15
0270-7306/08/$08.00+0     doi:10.1128/MCB.00178-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Physiological Role for Phosphatidic Acid in the Translocation of the Novel Protein Kinase C Apl II in Aplysia Neurons{triangledown}

Carole A. Farah, Ikue Nagakura, Daniel Weatherill, Xiaotang Fan, and Wayne S. Sossin*

Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec H3A 2B4, Canada

Received 4 February 2008/ Returned for modification 4 March 2008/ Accepted 17 May 2008

In Aplysia californica, the serotonin-mediated translocation of protein kinase C (PKC) Apl II to neuronal membranes is important for synaptic plasticity. The orthologue of PKC Apl II, PKC{varepsilon}, has been reported to require phosphatidic acid (PA) in conjunction with diacylglycerol (DAG) for translocation. We find that PKC Apl II can be synergistically translocated to membranes by the combination of DAG and PA. We identify a mutation in the C1b domain (arginine 273 to histidine; PKC Apl II-R273H) that removes the effects of exogenous PA. In Aplysia neurons, the inhibition of endogenous PA production by 1-butanol inhibited the physiological translocation of PKC Apl II by serotonin in the cell body and at the synapse but not the translocation of PKC Apl II-R273H. The translocation of PKC Apl II-R273H in the absence of PA was explained by two additional effects of this mutation: (i) the mutation removed C2 domain-mediated inhibition, and (ii) the mutation decreased the concentration of DAG required for PKC Apl II translocation. We present a model in which, under physiological conditions, PA is important to activate the novel PKC Apl II both by synergizing with DAG and removing C2 domain-mediated inhibition.

* Corresponding author. Mailing address: Department of Neurology and Neurosurgery, McGill University, Montreal Neurological Institute, BT 110, 3801 University Street, Montreal, Quebec H3A 2B4, Canada. Phone: (514) 398-1486. Fax: (514) 398-8106. E-Mail: wayne.sossin{at}mcgill.ca

{triangledown} Published ahead of print on 27 May 2008.

Molecular and Cellular Biology, August 2008, p. 4719-4733, Vol. 28, No. 15
0270-7306/08/$08.00+0     doi:10.1128/MCB.00178-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Farah, C. A., Weatherill, D., Dunn, T. W., Sossin, W. S. (2009). PKC Differentially Translocates during Spaced and Massed Training in Aplysia. J. Neurosci. 29: 10281-10286 [Abstract] [Full Text]  


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