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Molecular and Cellular Biology, September 2008, p. 5238-5250, Vol. 28, No. 17
0270-7306/08/$08.00+0     doi:10.1128/MCB.00747-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Two Subunits Specific to the PBAP Chromatin Remodeling Complex Have Distinct and Redundant Functions during Drosophila Development{triangledown} ,{dagger}

Inés Carrera,1,3 Jiri Zavadil,2 and Jessica E. Treisman1,3*

Kimmel Center for Biology and Medicine of the Skirball Institute,3 Department of Cell Biology,1 Department of Pathology, New York University School of Medicine, 540 First Avenue, New York, New York 100162

Received 8 May 2008/ Accepted 16 June 2008

Chromatin remodeling complexes control the availability of DNA binding sites to transcriptional regulators. Two distinct conserved forms of the SWI/SNF class of complexes are characterized by the presence of specific accessory subunits. In Drosophila, the core Brahma complex associates either with Osa to form the BAP complex or with Bap170 and Bap180 to form the PBAP complex. osa mutations reproduce only a subset of the developmental phenotypes caused by mutations in subunits of the core complex. To test whether the PBAP complex performs the remaining functions, we generated mutations in bap170 and bap180. Surprisingly, we found that Bap180 is not essential for viability, although it is required in ovarian follicle cells for normal eggshell development. Bap170 is necessary to stabilize the Bap180 protein, but a mutant form that retains this function is sufficient for both survival and fertility. The two subunits act redundantly to allow metamorphosis; using gene expression profiling of bap170 bap180 double mutants, we found that the PBAP complex regulates genes involved in tissue remodeling and immune system function. Finally, we generated mutants lacking Bap170, Bap180, and Osa in the germ line to demonstrate that the core Brahma complex can function in oogenesis without any of these accessory subunits.

* Corresponding author. Mailing address: Skirball Institute 4/9, New York University School of Medicine, 540 First Avenue, New York, NY 10016. Phone: (212) 263-1031. Fax: (212) 263-7760. E-mail: treisman{at}saturn.med.nyu.edu

{triangledown} Published ahead of print on 23 June 2008.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, September 2008, p. 5238-5250, Vol. 28, No. 17
0270-7306/08/$08.00+0     doi:10.1128/MCB.00747-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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