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Molecular and Cellular Biology, October 2008, p. 6022-6032, Vol. 28, No. 19
0270-7306/08/$08.00+0     doi:10.1128/MCB.00684-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

The T Body, a New Cytoplasmic RNA Granule in Saccharomyces cerevisiae{triangledown}

Francisco Malagon and Torben Heick Jensen*

Center for mRNP Biogenesis and Metabolism, Department of Molecular Biology, Aarhus University, C.F. Møllers Allé, Bldg. 130, DK-8000 Aarhus C, Denmark

Received 26 April 2008/ Returned for modification 29 May 2008/ Accepted 24 July 2008

A large share of mRNA processing and packaging events occurs cotranscriptionally. To explore the hypothesis that transcription defects may affect mRNA fate, we analyzed poly(A)+ RNA distribution in Saccharomyces cerevisiae strains harboring mutations in Rpb1p, the largest subunit of RNA polymerase II. In certain rpb1 mutants, a poly(A)+ RNA granule, distinct from any known structure, strongly accumulated in a confined space of the cytoplasm. RNA and protein expressed from Ty1 retrovirus-like elements colocalized with this new granule, which we have consequently named the T body. A visual screen revealed that the deletion of most genes with proposed functions in Ty1 biology unexpectedly does not alter T-body levels. In contrast, the deletion of genes encoding the Mediator transcription initiation factor subunits Srb2p and Srb5p as well as the Ty1 transcriptional regulator Spt21p greatly enhances T-body formation. Our data disclose a new cellular body putatively involved in the assembly of Ty1 particles and suggest that the cytoplasmic fate of mRNA can be affected by transcription initiation events.

* Corresponding author. Mailing address: Department of Molecular Biology, University of Aarhus, C.F. Møllers Allé, Building 1130, 8000 Århus C, Denmark. Phone: 45 8942 2609. Fax: 45 8619 6500. E-mail: thj{at}mb.au.dk

{triangledown} Published ahead of print on 4 August 2008.

Molecular and Cellular Biology, October 2008, p. 6022-6032, Vol. 28, No. 19
0270-7306/08/$08.00+0     doi:10.1128/MCB.00684-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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