This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Saberi, A.
Right arrow Articles by Sonoda, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Saberi, A.
Right arrow Articles by Sonoda, E.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, October 2008, p. 6113-6122, Vol. 28, No. 19
0270-7306/08/$08.00+0     doi:10.1128/MCB.00156-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

The 9-1-1 DNA Clamp Is Required for Immunoglobulin Gene Conversion{triangledown}

Alihossein Saberi,1,{dagger} Makoto Nakahara,1 Julian E. Sale,2 Koji Kikuchi,1 Hiroshi Arakawa,3 Jean-Marie Buerstedde,3 Kenichi Yamamoto,4 Shunichi Takeda,1 and Eiichiro Sonoda1*

Radiation Genetics, Graduate School of Medicine, Kyoto University, Yoshida-Konoe, Sakyo-ku, Kyoto 606-8501, Japan,1 Medical Research Council Laboratory of Molecular Biology, Division of Protein and Nucleic Acid Chemistry, Hills Road, Cambridge CB2 2QH, United Kingdom,2 GSF, Institute for Molecular Radiobiology, Ingolstaedter Landstr. 1, D-85764 Neuherberg-Munich, Germany,3 Molecular Pathology, Cancer Research Institute, Kanazawa University, Kanazawa, Ishikawa 920-0934, Japan4

Received 29 January 2008/ Returned for modification 15 April 2008/ Accepted 22 July 2008

Chicken DT40 cells deficient in the 9-1-1 checkpoint clamp exhibit hypersensitivity to a variety of DNA-damaging agents. Although recent work suggests that, in addition to its role in checkpoint activation, this complex may play a role in homologous recombination and translesion synthesis, the cause of this hypersensitivity has not been studied thoroughly. The immunoglobulin locus of DT40 cells allows monitoring of homologous recombination and translesion synthesis initiated by activation-induced deaminase (AID)-dependent abasic sites. We show that both the RAD9–/– and RAD17–/– mutants exhibit substantially reduced immunoglobulin gene conversion. However, the level of nontemplated immunoglobulin point mutation increased in these mutants, a finding that is reminiscent of the phenotype resulting from the loss of RAD51 paralogs or Brca2. This suggests that the 9-1-1 complex does not play a central role in translesion synthesis in this context. Despite reduced immunoglobulin gene conversion, the RAD9–/– and RAD17–/– cells do not exhibit a prominent defect in double-strand break-induced gene conversion or a sensitivity to camptothecin. This suggests that the roles of Rad9 and Rad17 may be confined to a subset of homologous recombination reactions initiated by replication-stalling lesions rather than those associated with double-strand break repair.

* Corresponding author. Mailing address: Department of Radiation Genetics, Kyoto University Graduate School of Medicine, Yoshida-konoe, Sakyo-ku, Kyoto 606-8501, Japan. Phone: 81-75-753-4410. Fax: 81-75-753-4419. E-mail: esonoda{at}rg.med.kyoto-u.ac.jp

{triangledown} Published ahead of print on 28 July 2008.

{dagger} Present address: Radiologic Technology, Ahwaz Jondishapoor University of Medical Sciences, Ahwaz, Iran.

Molecular and Cellular Biology, October 2008, p. 6113-6122, Vol. 28, No. 19
0270-7306/08/$08.00+0     doi:10.1128/MCB.00156-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kikuchi, K., Abdel-Aziz, H. I., Taniguchi, Y., Yamazoe, M., Takeda, S., Hirota, K. (2009). Bloom DNA Helicase Facilitates Homologous Recombination between Diverged Homologous Sequences. J. Biol. Chem. 284: 26360-26367 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»