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Molecular and Cellular Biology, January 2008, p. 656-665, Vol. 28, No. 2
0270-7306/08/$08.00+0     doi:10.1128/MCB.01531-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Global Role for Polyadenylation-Assisted Nuclear RNA Degradation in Posttranscriptional Gene Silencing{triangledown}

Shao-Win Wang,1,2* Abigail L. Stevenson,2,{dagger} Stephen E. Kearsey,2 Stephen Watt,3 and Jürg Bähler3

Division of Molecular and Genomic Medicine, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County 350, Taiwan,1 Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, United Kingdom,2 Fission Yeast Functional Genomics Group, Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, United Kingdom3

Received 22 August 2007/ Returned for modification 19 September 2007/ Accepted 2 November 2007

Fission yeast Cid14, a component of the TRAMP (Cid14/Trf4-Air1-Mtr4 polyadenylation) complex, polyadenylates nuclear RNA and stimulates degradation by the exosome for RNA quality control. Here, we analyze patterns of global gene expression in cells lacking the Cid14 or the Dis3/Rpr44 subunit of the nuclear exosome. We found that transcripts from many genes induced during meiosis, including key regulators, accumulated in the absence of Cid14 or Dis3. Moreover, our data suggest that additional substrates include transcripts involved in heterochromatin assembly. Mutant cells lacking Cid14 and/or Dis3 accumulate transcripts corresponding to naturally silenced repeat elements within heterochromatic domains, reflecting defects in centromeric gene silencing and derepression of subtelomeric gene expression. We also uncover roles for Cid14 and Dis3 in maintaining the genomic integrity of ribosomal DNA. Our data indicate that polyadenylation-assisted nuclear RNA turnover functions in eliminating a variety of RNA targets to control diverse processes, such as heterochromatic gene silencing, meiotic differentiation, and maintenance of genomic integrity.

* Corresponding author. Mailing address: Division of Molecular and Genomic Medicine, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County 350, Taiwan. Phone: 886 37 246166, ext. 35367. Fax: 886 37 586459. E-mail: shaowinwang{at}nhri.org.tw

{triangledown} Published ahead of print on 19 November 2007.

{dagger} Present address: Post-transcriptional Control Group, Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom.

Molecular and Cellular Biology, January 2008, p. 656-665, Vol. 28, No. 2
0270-7306/08/$08.00+0     doi:10.1128/MCB.01531-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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