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Molecular and Cellular Biology, November 2008, p. 6547-6556, Vol. 28, No. 21
0270-7306/08/$08.00+0     doi:10.1128/MCB.00906-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

A Direct Interaction between the Utp6 Half-a-Tetratricopeptide Repeat Domain and a Specific Peptide in Utp21 Is Essential for Efficient Pre-rRNA Processing{triangledown} ,{dagger}

Erica A. Champion,1 Bennett H. Lane,2 Meredith E. Jackrel,3 Lynne Regan,2,3 and Susan J. Baserga1,2,4*

Departments of Genetics,1 Molecular Biophysics and Biochemistry,2 Chemistry,3 Therapeutic Radiology, Yale University, New Haven, Connecticut 065204

Received 6 June 2008/ Returned for modification 5 August 2008/ Accepted 14 August 2008

The small subunit (SSU) processome is a ribosome biogenesis intermediate that assembles from its subcomplexes onto the pre-18S rRNA with yet unknown order and structure. Here, we investigate the architecture of the UtpB subcomplex of the SSU processome, focusing on the interaction between the half-a-tetratricopeptide repeat (HAT) domain of Utp6 and a specific peptide in Utp21. We present a comprehensive map of the interactions within the UtpB subcomplex and further show that the N-terminal domain of Utp6 interacts with Utp18 while the HAT domain interacts with Utp21. Using a panel of point and deletion mutants of Utp6, we show that an intact HAT domain is essential for efficient pre-rRNA processing and cell growth. Further investigation of the Utp6-Utp21 interaction using both genetic and biophysical methods shows that the HAT domain binds a specific peptide ligand in Utp21, the first example of a HAT domain peptide ligand, with a dissociation constant of 10 µM.

* Corresponding author. Mailing address: Department of Molecular Biophysics and Biochemistry, Yale School of Medicine, 333 Cedar Street P.O. Box 208040, C114 SHM, New Haven, CT 06520-8040. Phone: (203) 785-4618. Fax: (203) 785-6404. E-mail: susan.baserga{at}yale.edu

{triangledown} Published ahead of print on 25 August 2008.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, November 2008, p. 6547-6556, Vol. 28, No. 21
0270-7306/08/$08.00+0     doi:10.1128/MCB.00906-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Champion, E. A., Kundrat, L., Regan, L., Baserga, S. J. (2009). A structural model for the HAT domain of Utp6 incorporating bioinformatics and genetics. Protein Eng Des Sel 22: 431-439 [Abstract] [Full Text]  


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