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Molecular and Cellular Biology, February 2008, p. 1207-1217, Vol. 28, No. 4
0270-7306/08/$08.00+0     doi:10.1128/MCB.01069-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Different Requirements of the SWI/SNF Complex for Robust Nucleosome Displacement at Promoters of Heat Shock Factor and Msn2- and Msn4-Regulated Heat Shock Genes{triangledown}

Tamara Y. Erkina, Paul A. Tschetter, and Alexandre M. Erkine*

Division of Basic Biomedical Sciences, University of South Dakota, Sanford School of Medicine, Vermillion, South Dakota 57069

Received 15 June 2007/ Returned for modification 16 July 2007/ Accepted 23 November 2007

The stress response in yeast cells is regulated by at least two classes of transcription activators—HSF and Msn2/4, which differentially affect promoter chromatin remodeling. We demonstrate that the deletion of SNF2, an ATPase activity-containing subunit of the chromatin remodeling SWI/SNF complex, eliminates histone displacement, RNA polymerase II recruitment, and heat shock factor (HSF) binding at the HSP12 promoter while delaying these processes at the HSP82 and SSA4 promoters. Out of the three promoters, the double deletion of MSN2 and MSN4 eliminates both chromatin remodeling and HSF binding only at the HSP12 promoter, suggesting that Msn2/4 activators are primary determinants of chromatin disassembly at the HSP12 promoter. Unexpectedly, during heat shock the level of Msn2/4 at the HSP12 promoter declines. This is likely a result of promoter-targeted Msn2/4 degradation associated with transcription complex assembly. While histone displacement kinetic profiles bear clear promoter specificity, the kinetic profiles of recovery from heat shock for all analyzed genes display an equal or even higher nucleosome return rate, which is to some extent delayed by the deletion of SNF2.

* Corresponding author. Mailing address: Division of Basic Biomedical Sciences, University of South Dakota, Sanford School of Medicine, 414 E. Clark St., Vermillion, SD 57069. Phone: (605) 677-5164. Fax: (605) 677-6381. E-mail: aerkine{at}usd.edu

{triangledown} Published ahead of print on 10 December 2007.

Molecular and Cellular Biology, February 2008, p. 1207-1217, Vol. 28, No. 4
0270-7306/08/$08.00+0     doi:10.1128/MCB.01069-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kremer, S. B., Gross, D. S. (2009). SAGA and Rpd3 Chromatin Modification Complexes Dynamically Regulate Heat Shock Gene Structure and Expression. J. Biol. Chem. 284: 32914-32931 [Abstract] [Full Text]  


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