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Molecular and Cellular Biology, February 2008, p. 1373-1382, Vol. 28, No. 4
0270-7306/08/$08.00+0     doi:10.1128/MCB.01651-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

DNA Polymerase {delta} Is Preferentially Recruited during Homologous Recombination To Promote Heteroduplex DNA Extension{triangledown}

Laurent Maloisel,1* Francis Fabre,1 and Serge Gangloff1,2*

CEA, DSV, iRCM, SIGRR, LERA, Fontenay-aux-Roses F-92265, France,1 CNRS, UMR 217, Fontenay-aux-Roses F-92265, France2

Received 6 September 2007/ Returned for modification 26 October 2007/ Accepted 5 December 2007

DNA polymerases play a central role during homologous recombination (HR), but the identity of the enzyme(s) implicated remains elusive. The pol3-ct allele of the gene encoding the catalytic subunit of DNA polymerase {delta} (Pol{delta}) has highlighted a role for this polymerase in meiotic HR. We now address the ubiquitous role of Pol{delta} during HR in somatic cells. We find that pol3-ct affects gene conversion tract length during mitotic recombination whether the event is initiated by single-strand gaps following UV irradiation or by site-specific double-strand breaks. We show that the pol3-ct effects on gene conversion are completely independent of mismatch repair, indicating that shorter gene conversion tracts in pol3-ct correspond to shorter extensions of primed DNA synthesis. Interestingly, we find that shorter repair tracts do not favor synthesis-dependent strand annealing at the expense of double-strand-break repair. Finally, we show that the DNA polymerases that have been previously suspected to mediate HR repair synthesis (Pol{varepsilon} and Pol{eta}) do not affect gene conversion during induced HR, including in the pol3-ct background. Our results argue strongly for the preferential recruitment of Pol{delta} during HR.

* Corresponding author. Mailing address: CEA, DSV, iRCM, SIGRR, LERA, Fontenay-aux-Roses F-92265, France. Phone for Serge Gangloff: (33) 1 46 54 90 51. Fax: (33) 1 46 54 95 98. E-mail: serge.gangloff{at}cea.fr. Phone for Laurent Maloisel: (33) 1 46 54 90 53. Fax: (33) 1 46 54 95 98. E-mail: laurent.maloisel{at}cea.fr

{triangledown} Published ahead of print on 17 December 2007.

Molecular and Cellular Biology, February 2008, p. 1373-1382, Vol. 28, No. 4
0270-7306/08/$08.00+0     doi:10.1128/MCB.01651-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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