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CEA, IbiTec-S, Service de Biologie Intégrative et Génétique Moléculaire, Gif sur Yvette Cedex F-91191, France,1 Saitama Medical University, Department of Molecular Biology, 38 Morohongo, Moroyama, Iruma-Gun, Saitama 350-04, Japan,2 Organisation et Dynamique Nucléaire, LBME-CNRS, Université de Toulouse, 118 route de Narbonne, Toulouse F-31000, France3
Received 14 August 2007/ Returned for modification 24 September 2007/ Accepted 7 December 2007
Rpa34 and Rpa49 are nonessential subunits of RNA polymerase I, conserved in species from Saccharomyces cerevisiae and Schizosaccharomyces pombe to humans. Rpa34 bound an N-terminal region of Rpa49 in a two-hybrid assay and was lost from RNA polymerase in an rpa49 mutant lacking this Rpa34-binding domain, whereas rpa34
weakened the binding of Rpa49 to RNA polymerase. rpa34
mutants were caffeine sensitive, and the rpa34
mutation was lethal in a top1
mutant and in rpa14
, rpa135(L656P), and rpa135(D395N) RNA polymerase mutants. These defects were shared by rpa49
mutants, were suppressed by the overexpression of Rpa49, and thus, were presumably mediated by Rpa49 itself. rpa49 mutants lacking the Rpa34-binding domain behaved essentially like rpa34
mutants, but strains carrying rpa49
and rpa49-338::HIS3 (encoding a form of Rpa49 lacking the conserved C terminus) had reduced polymerase occupancy at 30°C, failed to grow at 25°C, and were sensitive to 6-azauracil and mycophenolate. Mycophenolate almost fully dissociated the mutant polymerase from its ribosomal DNA (rDNA) template. The rpa49
and rpa49-338::HIS3 mutations had a dual effect on the transcription initiation factor Rrn3 (TIF-IA). They partially impaired its recruitment to the rDNA promoter, an effect that was bypassed by an N-terminal deletion of the Rpa43 subunit encoded by rpa43-35,326, and they strongly reduced the release of the Rrn3 initiation factor during elongation. These data suggest a dual role of the Rpa49-Rpa34 dimer during the recruitment of Rrn3 and its subsequent dissociation from the elongating polymerase.
Published ahead of print on 17 December 2007.
Supplemental material for this article may be found at http://mcb.asm.org/.
| J. Bacteriol. | J. Virol. | Eukaryot. Cell |
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