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Molecular and Cellular Biology, March 2008, p. 1924-1935, Vol. 28, No. 6
0270-7306/08/$08.00+0     doi:10.1128/MCB.01647-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Long-Range Communication between the Silencers of HMR{triangledown}

Lourdes Valenzuela,1 Namrita Dhillon,1 Rudra N. Dubey,2 Marc R. Gartenberg,2 and Rohinton T. Kamakaka1*

Department of Pharmacology, 683 Hoes Lane, UMDNJ-Robert Wood Johnson Medical School, Piscataway, New Jersey 08854,2 Department of MCD Biology, Sinsheimer Labs, 1156 High Street, University of California, Santa Cruz, California 950641

Received 5 September 2007/ Returned for modification 2 October 2007/ Accepted 29 December 2007

Gene regulation involves long-range communication between silencers, enhancers, and promoters. In Saccharomyces cerevisiae, silencers flank transcriptionally repressed genes to mediate regional silencing. Silencers recruit the Sir proteins, which then spread along chromatin to encompass the entire silenced domain. In this report we have employed a boundary trap assay, an enhancer activity assay, chromatin immunoprecipitations, and chromosome conformation capture analyses to demonstrate that the two HMR silencer elements are in close proximity and functionally communicate with one another in vivo. We further show that silencing is necessary for these long-range interactions, and we present models for Sir-mediated silencing based upon these results.

* Corresponding author. Mailing address: Department of MCD Biology, Sinsheimer Labs, 1156 High Street, University of California, Santa Cruz, California 95064. Phone: (831) 459-3391. Fax: (831) 459-3139. E-mail: rohinton{at}biology.ucsc.edu

{triangledown} Published ahead of print on 14 January 2008.

Molecular and Cellular Biology, March 2008, p. 1924-1935, Vol. 28, No. 6
0270-7306/08/$08.00+0     doi:10.1128/MCB.01647-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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