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Molecular and Cellular Biology, May 2008, p. 3038-3044, Vol. 28, No. 9
0270-7306/08/$08.00+0     doi:10.1128/MCB.02064-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Degradation of a Polyadenylated rRNA Maturation By-Product Involves One of the Three RRP6-Like Proteins in Arabidopsis thaliana{triangledown}

Heike Lange, Sarah Holec, Valérie Cognat, Laurent Pieuchot, Monique Le Ret, Jean Canaday, and Dominique Gagliardi*

Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, Unité Propre de Recherche 2357, Conventionné avec l'Université Louis Pasteur, 67000 Strasbourg, France

Received 16 November 2007/ Returned for modification 20 December 2007/ Accepted 11 February 2008

Yeast Rrp6p and its human counterpart, PM/Scl100, are exosome-associated proteins involved in the degradation of aberrant transcripts and processing of precursors to stable RNAs, such as the 5.8S rRNA, snRNAs, and snoRNAs. The activity of yeast Rrp6p is stimulated by the polyadenylation of its RNA substrates. We identified three RRP6-like proteins in Arabidopsis thaliana: AtRRP6L3 is restricted to the cytoplasm, whereas AtRRP6L1 and -2 have different intranuclear localizations. Both nuclear RRP6L proteins are functional, since AtRRP6L1 complements the temperature-sensitive phenotype of a yeast rrp6{Delta} strain and mutation of AtRRP6L2 leads to accumulation of an rRNA maturation by-product. This by-product corresponds to the excised 5' part of the 18S-5.8S-25S rRNA precursor and accumulates as a polyadenylated transcript, suggesting that RRP6L2 is involved in poly(A)-mediated RNA degradation in plant nuclei. Interestingly, the rRNA maturation by-product is a substrate of AtRRP6L2 but not of AtRRP6L1. This result and the distinctive subcellular distribution of AtRRP6L1 to -3 indicate a specialization of RRP6-like proteins in Arabidopsis.

* Corresponding author. Mailing address: Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, Unité Propre de Recherche 2357, 12 rue du général Zimmer, 67000 Strasbourg, France. Phone: 33 3 88 41 71 62. Fax: 33 3 88 61 44 42. E-mail: dominique.gagliardi{at}ibmp-ulp.u-strasbg.fr

{triangledown} Published ahead of print on 19 February 2008.

Molecular and Cellular Biology, May 2008, p. 3038-3044, Vol. 28, No. 9
0270-7306/08/$08.00+0     doi:10.1128/MCB.02064-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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