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Molecular and Cellular Biology, January 2009, p. 150-156, Vol. 29, No. 1
0270-7306/09/$08.00+0     doi:10.1128/MCB.00997-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Weak but Uniform Enrichment of the Histone Variant macroH2A1 along the Inactive X Chromosome{triangledown}

Flore Mietton,1 Aditya K. Sengupta,2 Annie Molla,1 Gisele Picchi,4 Sophie Barral,1 Laurent Heliot,3 Thierry Grange,4 Anton Wutz,2 and Stefan Dimitrov1*

Institut Albert Bonniot, INSERM/UJF-U823, Site Santé-BP 170, 38042 Grenoble Cedex 9, France,1 Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria,2 Institut de Recherche Interdisciplinaire, FRE 2963 CNRS/Université de Lille I/Université de Lille II, Lille, France,3 Institut Jacques Monod, CNRS, Universités Paris 6 et 7, Tour 43, 2 Place Jussieu, 75251 Paris Cedex 06, France4

Received 24 June 2008/ Returned for modification 4 August 2008/ Accepted 10 October 2008

We studied the enrichment and distribution of the histone variant mH2A1 in the condensed inactive X (Xi) chromosome. By using highly specific antibodies against mH2A1 and stable HEK 293 cell lines expressing either green fluorescent protein (GFP)-mH2A1 or GFP-H2A, we found that the Xi chromosome contains ~1.5-fold more mH2A1 than the autosomes. To determine the in vivo distribution of mH2A1 along the X chromosome, we used a native chromatin immunoprecipitation-on-chip technique. DNA isolated from mH2A1-immunoprecipitated nucleosomes from either male or female mouse liver were hybridized to tiling microarrays covering 5 kb around most promoters or the entire X chromosome. The data show that mH2A1 is uniformly distributed across the entire Xi chromosome. Interestingly, a stronger mH2A1 enrichment along the pseudoautosomal X chromosome region was observed in both sexes. Our results indicate a potential role for macroH2A in large-scale chromosome structure and genome stability.

* Corresponding author. Mailing address: Institut Albert Bonniot, INSERM/UJF-U823, Site Santé-BP 170, 38042 Grenoble Cedex 9, France. Phone: (33) 4 76 54 94 73. Fax: (33) 4 76 54 95 95. E-mail: stefan.dimitrov{at}ujf-grenoble.fr

{triangledown} Published ahead of print on 20 October 2008.

Molecular and Cellular Biology, January 2009, p. 150-156, Vol. 29, No. 1
0270-7306/09/$08.00+0     doi:10.1128/MCB.00997-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Marks, H., Chow, J. C., Denissov, S., Francoijs, K.-J., Brockdorff, N., Heard, E., Stunnenberg, H. G. (2009). High-resolution analysis of epigenetic changes associated with X inactivation. Genome Res 19: 1361-1373 [Abstract] [Full Text]  


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