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Molecular and Cellular Biology, July 2009, p. 3791-3802, Vol. 29, No. 13
0270-7306/09/$08.00+0     doi:10.1128/MCB.01865-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Spatially Restricted Translation of the xCR1 mRNA in Xenopus Embryos{triangledown}

Yan Zhang,1 Kara D. Forinash,1 Jered McGivern,1 Brian Fritz,1 Karel Dorey,2 and Michael D. Sheets1*

University of Wisconsin School of Medicine and Public Health, Department of Biomolecular Chemistry, 1300 University Avenue, Madison, Wisconsin 53706,1 University of Manchester, Healing Foundation Centre, Oxford Road, M13 9PT Manchester, United Kingdom2

Received 6 December 2008/ Returned for modification 8 February 2009/ Accepted 4 April 2009

The xCR1 protein is a maternal determinant and cofactor for nodal signaling in vertebrate embryos. The xCR1 protein accumulates specifically in the animal cells of Xenopus embryos, but maternal xCR1 mRNA is distributed equally throughout all embryonic cells. Here, we show that vegetal cell-specific translational repression of xCR1 mRNA contributes to this spatially restricted accumulation of the xCR1 protein in Xenopus embryos. xCR1 mRNA was associated with polyribosomes in animal cells but not vegetal cells. A 351-nucleotide region of xCR1 mRNA's 3' untranslated region was sufficient to confer a spatially restricted pattern of translation to a luciferase reporter mRNA by repressing translation in vegetal cells. Repression depended upon the mRNA's 5' cap but not its 3' poly(A) tail. Furthermore, the region of xCR1 mRNA sufficient to confer vegetal cell-specific repression contained both Pumilio binding elements (PBEs) and binding sites for the CUG-BP1 protein. The PBEs and the CUG-BP1 sites were necessary but not sufficient for translation repression. Our studies of xCR1 mRNA document the first example of spatially regulated translation in controlling the asymmetric distribution of a maternal determinant in vertebrates.

* Corresponding author. Mailing address: University of Wisconsin—Madison, Department of Biomolecular Chemistry, 1300 University Avenue, Madison, WI 53706. Phone: (608) 262-9452. Fax: (608) 262-5253. E-mail: mdsheets{at}wisc.edu

{triangledown} Published ahead of print on 13 April 2009.

Molecular and Cellular Biology, July 2009, p. 3791-3802, Vol. 29, No. 13
0270-7306/09/$08.00+0     doi:10.1128/MCB.01865-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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