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Molecular and Cellular Biology, July 2009, p. 4014-4023, Vol. 29, No. 14
0270-7306/09/$08.00+0     doi:10.1128/MCB.01582-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Functional Redundancy of Yeast Proteins Reh1 and Rei1 in Cytoplasmic 60S Subunit Maturation{triangledown}

K. Mark Parnell* and Brenda L. Bass*

Department of Biochemistry and Howard Hughes Medical Institute, University of Utah School of Medicine, 15 North Medical Drive East, Room 4800, Salt Lake City, Utah 84112-5650

Received 8 October 2008/ Returned for modification 8 December 2008/ Accepted 1 May 2009

The biogenesis of the large (60S) ribosomal subunit in eukaryotes involves nucleolar, nucleoplasmic, and cytoplasmic steps. The cytoplasmic protein Rei1, found in all eukaryotes, was previously shown to be necessary for the nuclear reimport of 60S subunit export factor Arx1. In this study we investigate the function of Reh1, a protein with high sequence similarity to Rei1. We demonstrate an overlapping function for Reh1 and Rei1 in the cytoplasmic maturation of the 60S subunit that is independent of Arx1 recycling. We observe that strains lacking both Reh1 and Rei1 accumulate salt-labile 60S subunits, suggesting that Reh1/Rei1 is necessary for the cytoplasmic 60S subunit to adopt its mature, stable form.

* Corresponding author. Mailing address: Department of Biochemistry and Howard Hughes Medical Institute, University of Utah School of Medicine, 15 N Medical Drive East, Room 4800, Salt Lake City, UT 84112-5650. Phone for K. Mark Parnell: (801) 931-2420. Fax: (801) 746-5633. E-mail: markparnell1{at}gmail.com. Phone for Brenda L. Bass: (801) 581-4884. Fax: (801) 581-3824. E-mail: bbass{at}biochem.utah.edu

{triangledown} Published ahead of print on 11 May 2009.

Molecular and Cellular Biology, July 2009, p. 4014-4023, Vol. 29, No. 14
0270-7306/09/$08.00+0     doi:10.1128/MCB.01582-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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