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Molecular and Cellular Biology, August 2009, p. 4057-4066, Vol. 29, No. 15
0270-7306/09/$08.00+0     doi:10.1128/MCB.00400-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

SWI/SNF and Asf1p Cooperate To Displace Histones during Induction of the Saccharomyces cerevisiae HO Promoter{triangledown} ,{dagger}

Triantafyllos Gkikopoulos, Kristina M. Havas,{ddagger} Hilary Dewar, and Tom Owen-Hughes*

Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom

Received 27 March 2009/ Accepted 15 May 2009

Regulation of the Saccharomyces cerevisiae HO promoter has been shown to require the recruitment of chromatin-modifying and -remodeling enzymes. Despite this, relatively little is known about what changes to chromatin structure occur during the course of regulation at HO. Here, we used indirect end labeling in synchronized cultures to show that the chromatin structure is disrupted in a region that spans bp –600 to –1800 relative to the transcriptional start site. Across this region, there is a loss of canonical nucleosomes and a reduction in histone DNA cross-linking, as monitored by chromatin immunoprecipitation. The ATPase Snf2 is required for these alterations, but the histone acetyltransferase Gcn5 is not. This suggests that the SWI/SNF complex is directly involved in nucleosome removal at HO. We also present evidence indicating that the histone chaperone Asf1 assists in this. These observations suggest that SWI/SNF-related complexes in concert with histone chaperones act to remove histone octamers from DNA during the course of gene regulation.

* Corresponding author. Mailing address: Wellcome Trust Center for Gene Regulation and Expression, College of Life Sciences University of Dundee, Dundee DD1 5EH, United Kingdom. Phone: (44) 1382-385796. Fax: (44) 1382-388072. E-mail: t.a.owenhughes{at}dundee.ac.uk

{triangledown} Published ahead of print on 26 May 2009.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

{ddagger} Present address: Unità di Patologia Oncologica, CeSI Università G. D’ Annunzio, Via Colle dell’ Ara, 66013 Chieti Scalo, Chieti, Italy.

Molecular and Cellular Biology, August 2009, p. 4057-4066, Vol. 29, No. 15
0270-7306/09/$08.00+0     doi:10.1128/MCB.00400-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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