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Molecular and Cellular Biology, September 2009, p. 4595-4603, Vol. 29, No. 17
0270-7306/09/$08.00+0     doi:10.1128/MCB.00275-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

A Randomly Integrated Transgenic H19 Imprinting Control Region Acquires Methylation Imprinting Independently of Its Establishment in Germ Cells{triangledown}

Hitomi Matsuzaki, Eiichi Okamura, Motoshi Shimotsuma, Akiyoshi Fukamizu, and Keiji Tanimoto*

Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japan

Received 3 March 2009/ Returned for modification 4 April 2009/ Accepted 13 June 2009

The imprinted expression of the mouse Igf2/H19 locus is governed by the differential methylation of the imprinting control region (ICR), which is established initially in germ cells and subsequently maintained in somatic cells, depending on its parental origin. By grafting a 2.9-kbp H19 ICR fragment into a human β-globin yeast artificial chromosome in transgenic mice, we previously showed that the ICR could recapitulate imprinted methylation and expression at a heterologous locus, suggesting that the H19 ICR in the β-globin locus contained sufficient information to maintain the methylation mark (K. Tanimoto, M. Shimotsuma, H. Matsuzaki, A. Omori, J. Bungert, J. D. Engel, and A. Fukamizu, Proc. Natl. Acad. Sci. USA 102:10250-10255, 2005). Curiously, however, the transgenic H19 ICR was not methylated in sperm, which was distinct from that seen in the endogenous locus. Here, we reevaluated the ability of the H19 ICR to mark the parental origin using more rigid criteria. In the testis, the methylation levels of the solitary 2.9-kbp transgenic ICR fragment varied significantly between six transgenic mouse lines. However, in somatic cells, the paternally inherited ICR fragment exhibited consistently higher methylation levels at five out of six randomly integrated sites in the mouse genome. These results clearly demonstrated that the H19 ICR could acquire parent-of-origin-dependent methylation after fertilization independently of the chromosomal integration site or the prerequisite methylation acquisition in male germ cells.

* Corresponding author. Mailing address: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, Ibaraki 305-8577, Japan. Phone and fax: (81) 29-853-6070. E-mail: keiji{at}tara.tsukuba.ac.jp

{triangledown} Published ahead of print on 22 June 2009.

Molecular and Cellular Biology, September 2009, p. 4595-4603, Vol. 29, No. 17
0270-7306/09/$08.00+0     doi:10.1128/MCB.00275-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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