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Molecular and Cellular Biology, October 2009, p. 5590-5603, Vol. 29, No. 20
0270-7306/09/$08.00+0     doi:10.1128/MCB.00039-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Drosophila Suppressor of Sable Protein [Su(s)] Promotes Degradation of Aberrant and Transposon-Derived RNAs{triangledown}

Yung-Shu Kuan,{dagger} Paul Brewer-Jensen, Wen-Li Bai,# Cedric Hunter, Carrie B. Wilson, Sarah Bass, John Abernethy, James S. Wing, and Lillie L. Searles*

Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-3280

Received 9 January 2009/ Returned for modification 16 February 2009/ Accepted 9 August 2009

RNA-binding proteins act at various stages of gene expression to regulate and fine-tune patterns of mRNA accumulation. One protein in this class is Drosophila Su(s), a nuclear protein that has been previously shown to inhibit the accumulation of mutant transcripts by an unknown mechanism. Here, we have identified several additional RNAs that are downregulated by Su(s). These Su(s) targets include cryptic wild-type transcripts from the developmentally regulated Sgs4 and ng1 genes, noncoding RNAs derived from tandemly repeated {alpha}β/{alpha}{gamma} elements within an Hsp70 locus, and aberrant transcripts induced by Hsp70 promoter transgenes inserted at ectopic sites. We used the {alpha}β RNAs to investigate the mechanism of Su(s) function and obtained evidence that these transcripts are degraded by the nuclear exosome and that Su(s) promotes this process. Furthermore, we showed that the RNA binding domains of Su(s) are important for this effect and mapped the sequences involved to a 267-nucleotide region of an {alpha}β element. Taken together, these results suggest that Su(s) binds to certain nascent transcripts and stimulates their degradation by the nuclear exosome.

* Corresponding author. Mailing address: Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3280. Phone: (919) 966-4989. Fax: (919) 962-1625. E-mail: lsearles{at}email.unc.edu

{triangledown} Published ahead of print on 17 August 2009.

{dagger} Present address: Institute of Biochemical Science, National Taiwan University, Taipei, Taiwan.

# Present address: Dept. of Cancer Biology and Pharmocology, DUMC 3813, Duke University Medical Center, Durham, NC.

Molecular and Cellular Biology, October 2009, p. 5590-5603, Vol. 29, No. 20
0270-7306/09/$08.00+0     doi:10.1128/MCB.00039-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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