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Molecular and Cellular Biology, March 2009, p. 1116-1122, Vol. 29, No. 5
0270-7306/09/$08.00+0     doi:10.1128/MCB.01521-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

The C-Terminal Domain of Cernunnos/XLF Is Dispensable for DNA Repair In Vivo{triangledown} ,{dagger}

Laurent Malivert,1,2 Isabelle Callebaut,3 Paola Rivera-Munoz,1,2 Alain Fischer,1,4,5 Jean-Paul Mornon,3 Patrick Revy,1,4 and Jean-Pierre de Villartay1,4,5*

INSERM, Hôpital Necker-Enfants Malades, U768, Unité de Développement Normal et Pathologique du Système Immunitaire,1 Assistance Publique-Hôpitaux de Paris, Hôpital Necker-Enfants Malades, Unité d'Immunologie et d'Hématologie, F-75015 Paris,5 Université Paris Diderot—Paris 7, Bâtiment les Grands Moulins, 16 rue Marguerite Duras, 75205 Paris Cedex 13,2 Département de Biologie Structurale, IMPMC, CNRS UMR7590, Universités Paris 6 et 7,3 Université Paris-Descartes, Faculté de Médecine René Descartes, F-75005 Paris, France4

Received 30 September 2008/ Returned for modification 10 October 2008/ Accepted 9 December 2008

The core nonhomologous end-joining DNA repair pathway is composed of seven factors: Ku70, Ku80, DNA-PKcs, Artemis, XRCC4 (X4), DNA ligase IV (L4), and Cernunnos/XLF (Cernunnos). Although Cernunnos and X4 are structurally related and participate in the same complex together with L4, they have distinct functions during DNA repair. L4 relies on X4 but not on Cernunnos for its stability, and L4 is required for optimal interaction of Cernunnos with X4. We demonstrate here, using in vitro-generated Cernunnos mutants and a series of functional assays in vivo, that the C-terminal region of Cernunnos is dispensable for its activity during DNA repair.

* Corresponding author. Mailing address: INSERM U768, Hopital Necker-Enfants Malades, 149, rue de Sevres, 75015 Paris, France. Phone: 33 (0) 1 44 49 50 81. Fax: 33 (0) 1 42 73 06 40. E-mail: devillar{at}necker.fr

{triangledown} Published ahead of print on 22 December 2008.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, March 2009, p. 1116-1122, Vol. 29, No. 5
0270-7306/09/$08.00+0     doi:10.1128/MCB.01521-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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