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Molecular and Cellular Biology, March 2009, p. 1401-1410, Vol. 29, No. 6
0270-7306/09/$08.00+0     doi:10.1128/MCB.01643-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

The H3K4 Demethylase Lid Associates with and Inhibits Histone Deacetylase Rpd3{triangledown}

Nara Lee,1,2 Hediye Erdjument-Bromage,3 Paul Tempst,3 Richard S. Jones,4 and Yi Zhang1,2*

Howard Hughes Medical Institute,1 Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7295,2 Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 10021,3 Department of Biological Sciences, Southern Methodist University, Dallas, Texas 752754

Received 21 October 2008/ Returned for modification 17 November 2008/ Accepted 19 December 2008

JmjC domain-containing proteins have been shown to possess histone demethylase activity. One of these proteins is the Drosophila histone H3 lysine 4 demethylase Little imaginal discs (Lid), which has been genetically classified as a Trithorax group protein. However, contrary to the supposed function of Lid in gene activation, the biochemical activity of this protein entails the removal of a histone mark that is correlated with active transcription. To understand the molecular mechanism behind the function of Lid, we have purified a Lid-containing protein complex from Drosophila embryo nuclear extracts. In addition to Lid, the complex contains Rpd3, CG3815/Drosophila Pf1, CG13367, and Mrg15. Rpd3 is a histone deacetylase, and along with Polycomb group proteins, which antagonize the function of Trithorax group proteins, it negatively regulates transcription. By reconstituting the Lid complex, we demonstrated that the demethylase activity of Lid is not affected by its association with other proteins. However, the deacetylase activity of Rpd3 is greatly diminished upon incorporation into the Lid complex. Thus, our finding that Lid antagonizes Rpd3 function provides an explanation for the genetic classification of Lid as a positive transcription regulator.

* Corresponding author. Mailing address: Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7295. Phone: (919) 843-8225. Fax: (919) 966-4330. E-mail: yi_zhang{at}med.unc.edu

{triangledown} Published ahead of print on 29 December 2008.

Molecular and Cellular Biology, March 2009, p. 1401-1410, Vol. 29, No. 6
0270-7306/09/$08.00+0     doi:10.1128/MCB.01643-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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