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Molecular and Cellular Biology, April 2009, p. 1671-1681, Vol. 29, No. 7
0270-7306/09/$08.00+0     doi:10.1128/MCB.01182-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Ctp1CtIP and Rad32Mre11 Nuclease Activity Are Required for Rec12Spo11 Removal, but Rec12Spo11 Removal Is Dispensable for Other MRN-Dependent Meiotic Functions {triangledown}

Edgar Hartsuiker,1* Kenichi Mizuno,1 Monika Molnar,2 Juerg Kohli,2 Kunihiro Ohta,3 and Antony M. Carr1

Genome Damage and Stability Centre, Sussex University, Brighton BN1 9RQ, United Kingdom,1 Institute of Cell Biology, Baltzerstrasse 4, CH-3012 Bern, Switzerland,2 Department of Life Sciences, University of Tokyo, Komaba 3-8-1, Meguro-ku, Tokyo 153-8902, Japan3

Received 28 July 2008/ Returned for modification 18 August 2008/ Accepted 5 January 2009

The evolutionarily conserved Mre11/Rad50/Nbs1 (MRN) complex is involved in various aspects of meiosis. Whereas available evidence suggests that the Mre11 nuclease activity might be responsible for Spo11 removal in Saccharomyces cerevisiae, this has not been confirmed experimentally. This study demonstrates for the first time that Mre11 (Schizosaccharomyces pombe Rad32Mre11) nuclease activity is required for the removal of Rec12Spo11. Furthermore, we show that the CtIP homologue Ctp1 is required for Rec12Spo11 removal, confirming functional conservation between Ctp1CtIP and the more distantly related Sae2 protein from Saccharomyces cerevisiae. Finally, we show that the MRN complex is required for meiotic recombination, chromatin remodeling at the ade6-M26 recombination hot spot, and formation of linear elements (which are the equivalent of the synaptonemal complex found in other eukaryotes) but that all of these functions are proficient in a rad50S mutant, which is deficient for Rec12Spo11 removal. These observations suggest that the conserved role of the MRN complex in these meiotic functions is independent of Rec12Spo11 removal.

* Corresponding author. Mailing address: Genome Damage and Stability Centre, Sussex University, Brighton BN15 8HG, United Kingdom. Phone: 44 1273 873118. Fax: 44 1273 678121. E-mail: E.Hartsuiker{at}sussex.ac.uk

{triangledown} Published ahead of print on 12 January 2009.

Molecular and Cellular Biology, April 2009, p. 1671-1681, Vol. 29, No. 7
0270-7306/09/$08.00+0     doi:10.1128/MCB.01182-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Milman, N., Higuchi, E., Smith, G. R. (2009). Meiotic DNA Double-Strand Break Repair Requires Two Nucleases, MRN and Ctp1, To Produce a Single Size Class of Rec12 (Spo11)-Oligonucleotide Complexes. Mol. Cell. Biol. 29: 5998-6005 [Abstract] [Full Text]  


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