This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Smith, G E Articles by Fraser, M J Search for Related Content PubMed PubMed Citation Articles by Smith, G E Articles by Fraser, M J

Production of human beta interferon in insect cells infected with a baculovirus expression vector.

ABSTRACT

Autographa californica nuclear polyhedrosis virus (AcNPV) was used as an expression vector for human beta interferon. By using specially constructed plasmids, the protein-coding sequences for interferon were linked to the AcNPV promoter for the gene encoding for polyhedrin, the major occlusion protein. The interferon gene was inserted at various locations relative to the AcNPV polyhedrin transcriptional and translational signals, and the interferon-polyhedrin hybrid genes were transferred to infectious AcNPV expression vectors. Biologically active interferon was produced, and greater than 95% was secreted from infected insect cells. A maximum of ca. 5 X 10(6) U of interferon activity was produced by 10(6) infected cells. These results demonstrate that AcNPV should be suitable for use as a eucaryotic expression vector for the production of products from cloned genes.

This article has been cited by other articles:

• Jordan, K. R., McMahan, R. H., Oh, J. Z., Pipeling, M. R., Pardoll, D. M., Kedl, R. M., Kappler, J. W., Slansky, J. E. (2008). Baculovirus-Infected Insect Cells Expressing Peptide-MHC Complexes Elicit Protective Antitumor Immunity. J. Immunol. 180: 188-197 [Abstract] [Full Text]  
• Miyake, R., Kawamoto, J., Wei, Y.-L., Kitagawa, M., Kato, I., Kurihara, T., Esaki, N. (2007). Construction of a Low-Temperature Protein Expression System Using a Cold-Adapted Bacterium, Shewanella sp. Strain Ac10, as the Host. Appl. Environ. Microbiol. 73: 4849-4856 [Abstract] [Full Text]  
• Lin, C. H., Platt, M. D., Ficarro, S. B., Hoofnagle, M. H., Shabanowitz, J., Comai, L., Hunt, D. F., Owens, G. K. (2007). Mass spectrometric identification of phosphorylation sites of rRNA transcription factor upstream binding factor. Am. J. Physiol. Cell Physiol. 292: C1617-C1624 [Abstract] [Full Text]  
• Cui, M., Li, W., Liu, W., Yang, K., Pang, Y., Haoran, L. (2007). Production of Recombinant Orange-Spotted Grouper (Epinephelus coioides) Luteinizing Hormone in Insect Cells by the Baculovirus Expression System and Its Biological Effect. Biol. Reprod. 76: 74-84 [Abstract] [Full Text]  
• Jiang, S. S., Chang, I-S., Huang, L.-W., Chen, P.-C., Wen, C.-C., Liu, S.-C., Chien, L.-C., Lin, C.-Y., Hsiung, C. A., Juang, J.-L. (2006). Temporal Transcription Program of Recombinant Autographa californica Multiple Nucleopolyhedrosis Virus.. J. Virol. 80: 8989-8999 [Abstract] [Full Text]  
• Airenne, K. J., Peltomaa, E., Hytonen, V. P., Laitinen, O. H., Yla-Herttuala, S. (2003). Improved generation of recombinant baculovirus genomes in Escherichia coli. Nucleic Acids Res 31: e101-e101 [Abstract] [Full Text]  
• Aumiller, J. J., Hollister, J. R., Jarvis, D. L. (2003). A transgenic insect cell line engineered to produce CMP-sialic acid and sialylated glycoproteins. Glycobiology 13: 497-507 [Abstract] [Full Text]  
• Song, S. U., Shin, S.-H., Kim, S.-K., Choi, G.-S., Kim, W.-C., Lee, M.-H., Kim, S.-J., Kim, I.-H., Choi, M.-S., Hong, Y.-J., Lee, K.-H. (2003). Effective transduction of osteogenic sarcoma cells by a baculovirus vector. J. Gen. Virol. 84: 697-703 [Abstract] [Full Text]  
• Zhao, Y., Chapman, D. A. G., Jones, I. M. (2003). Improving baculovirus recombination. Nucleic Acids Res 31: e6-e6 [Abstract] [Full Text]  
• Chen, Y., Riley, D. J., Zheng, L., Chen, P.-L., Lee, W.-H. (2002). Phosphorylation of the Mitotic Regulator Protein Hec1 by Nek2 Kinase Is Essential for Faithful Chromosome Segregation. J. Biol. Chem. 277: 49408-49416 [Abstract] [Full Text]  
• Lo, H.-R., Chou, C.-C., Wu, T.-Y., Yuen, J. P.-Y., Chao, Y.-C. (2002). Novel Baculovirus DNA Elements Strongly Stimulate Activities of Exogenous and Endogenous Promoters. J. Biol. Chem. 277: 5256-5264 [Abstract] [Full Text]  
• Croizier, L., Jousset, F.-X., Veyrunes, J.-C., López-Ferber, M., Bergoin, M., Croizier, G. (2000). Protein requirements for assembly of virus-like particles of Junonia coenia densovirus in insect cells. J. Gen. Virol. 81: 1605-1613 [Abstract] [Full Text]  
• Wu, Y., Liu, G., Carstens, E. B. (1999). Replication, Integration, and Packaging of Plasmid DNA following Cotransfection with Baculovirus Viral DNA. J. Virol. 73: 5473-5480 [Abstract] [Full Text]  
• Volkman, L. (1995). Baculovirus bounty. Science 269: 1834-1834  
• Tang, L., Gounaris, K., Griffiths, C., Selkirk, M. E. (1995). Heterologous Expression and Enzymatic Properties of a Selenium-independent Glutathione Peroxidase from the Parasitic Nematode Brugia pahangi. J. Biol. Chem. 270: 18313-18318 [Abstract] [Full Text]  
• Mohr, I., Clark, R, Sun, S, Androphy, E., MacPherson, P, Botchan, M. (1990). Targeting the E1 replication protein to the papillomavirus origin of replication by complex formation with the E2 transactivator. Science 250: 1694-1699 [Abstract]  
• Odenwald, W F, Garbern, J, Arnheiter, H, Tournier-Lasserve, E, Lazzarini, R A (1989). The Hox-1.3 homeo box protein is a sequence-specific DNA-binding phosphoprotein.. Genes Dev. 3: 158-172 [Abstract]