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Multiple, Tandem Plasmid Integration in Saccharomyces cerevisiae

¹ Dana-Farber Cancer Institute and Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts 02115

ABSTRACT

Nonreplicating plasmids transform Saccharomyces cerevisiae by recombining with a homologous site in the genome. Frequently, multiple copies of the plasmid integrate in a tandem array. We show that, after transformation with restriction enzyme-cut plasmids, most, if not all, multimers arise by sequential integration of plasmid molecules into the same genomic location.