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Identification of the Cloned Gene for the Murine Transplantation Antigen H-2K^b by Hybridization with Synthetic Oligonucleotides

¹ Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461
² Memorial Sloan-Kettering Cancer Center, New York, New York 10021
³ Department of Molecular Genetics, City of Hope Research Institute, Duarte, California 91010

ABSTRACT

The H-2K^b gene is a member of the large major histocompatibility complex class I gene family. Since many members of this family cross-hybridize with class I cDNA probes, the cloned H-2K^b gene was identified by hybridization with specific oligonucleotide probes. This clone was definitively shown to encode the H-2K^b polypeptide by partial DNA sequencing and by serological and tryptic peptide analyses of the expressed product.