This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Celenza, J L Articles by Carlson, M Search for Related Content PubMed PubMed Citation Articles by Celenza, J L Articles by Carlson, M

Cloning and genetic mapping of SNF1, a gene required for expression of glucose-repressible genes in Saccharomyces cerevisiae.

ABSTRACT

A functional SNF1 gene product is required to derepress expression of many glucose-repressible genes in Saccharomyces cerevisiae. Strains carrying a snf1 mutation are unable to grow on sucrose, galactose, maltose, melibiose, or nonfermentable carbon sources; utilization of these carbon sources is regulated by glucose repression. The inability of snf1 mutants to utilize sucrose results from failure to derepress expression of the structural gene for invertase at the RNA level. We isolated recombinant plasmids carrying the SNF1 gene by complementation of the snf1 defect in S. cerevisiae. A 3.5-kilobase region is common to the DNA segments cloned in five different plasmids. Transformation of S. cerevisiae with an integrating vector carrying a segment of the cloned DNA resulted in integration of the plasmid at the SNF1 locus. This result indicates that the cloned DNA is homologous to sequences at the SNF1 locus. By mapping a plasmid marker linked to SNF1 in this transformant, we showed that the SNF1 gene is located on chromosome IV. We then mapped snf1 to a position 5.6 centimorgans distal to rna3 on the right arm; snf1 is not extremely closely linked to any previously mapped mutation.

This article has been cited by other articles:

• Santangelo, G. M. (2006). Glucose Signaling in Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 70: 253-282 [Abstract] [Full Text]  
• van Oevelen, C. J. C., van Teeffelen, H. A. A. M., van Werven, F. J., Timmers, H. Th. M. (2006). Snf1p-dependent Spt-Ada-Gcn5-acetyltransferase (SAGA) Recruitment and Chromatin Remodeling Activities on the HXT2 and HXT4 Promoters. J. Biol. Chem. 281: 4523-4531 [Abstract] [Full Text]  
• Boukaba, A., Georgieva, E. I., Myers, F. A., Thorne, A. W., Lopez-Rodas, G., Crane-Robinson, C., Franco, L. (2004). A Short-range Gradient of Histone H3 Acetylation and Tup1p Redistribution at the Promoter of the Saccharomyces cerevisiae SUC2 Gene. J. Biol. Chem. 279: 7678-7684 [Abstract] [Full Text]  
• Dong, L., Xu, C. W. (2004). Carbohydrates Induce Mono-ubiquitination of H2B in Yeast. J. Biol. Chem. 279: 1577-1580 [Abstract] [Full Text]  
• Hohmann, S. (2002). Osmotic Stress Signaling and Osmoadaptation in Yeasts. Microbiol. Mol. Biol. Rev. 66: 300-372 [Abstract] [Full Text]  
• Ashrafi, K., Lin, S. S., Manchester, J. K., Gordon, J. I. (2000). Sip2p and its partner Snf1p kinase affect aging in S. cerevisiae. Genes Dev. 14: 1872-1885 [Abstract] [Full Text]  
• Bonner, J. J., Carlson, T., Fackenthal, D. L., Paddock, D., Storey, K., Lea, K. (2000). Complex Regulation of the Yeast Heat Shock Transcription Factor. Mol. Biol. Cell 11: 1739-1751 [Abstract] [Full Text]  
• Tonukari, N. J., Scott-Craig, J. S., Walton, J. D. (2000). The Cochliobolus carbonum SNF1 Gene Is Required for Cell Wall-Degrading Enzyme Expression and Virulence on Maize. Plant Cell 12: 237-248 [Abstract] [Full Text]  
• Dombek, K. M., Voronkova, V., Raney, A., Young, E. T. (1999). Functional Analysis of the Yeast Glc7-Binding Protein Reg1 Identifies a Protein Phosphatase Type 1-Binding Motif as Essential for Repression of ADH2 Expression. Mol. Cell. Biol. 19: 6029-6040 [Abstract] [Full Text]  
• Ozcan, S., Johnston, M. (1999). Function and Regulation of Yeast Hexose Transporters. Microbiol. Mol. Biol. Rev. 63: 554-569 [Abstract] [Full Text]  
• Davies, J. P., Yildiz, F. H., Grossman, A. R. (1999). Sac3, an Snf1-like Serine/Threonine Kinase That Positively and Negatively Regulates the Responses of Chlamydomonas to Sulfur Limitation. Plant Cell 11: 1179-1190 [Abstract] [Full Text]  
• Park, S. H., Koh, S. S., Chun, J. H., Hwang, H. J., Kang, H. S. (1999). Nrg1 Is a Transcriptional Repressor for Glucose Repression of STA1 Gene Expression in Saccharomyces cerevisiae. Mol. Cell. Biol. 19: 2044-2050 [Abstract] [Full Text]  
• Lutfiyya, L. L., Iyer, V. R., DeRisi, J., DeVit, M. J., Brown, P. O., Johnston, M. (1998). Characterization of Three Related Glucose Repressors and Genes They Regulate in Saccharomyces cerevisiae. Genetics 150: 1377-1391 [Abstract] [Full Text]  
• Treitel, M. A., Kuchin, S., Carlson, M. (1998). Snf1 Protein Kinase Regulates Phosphorylation of the Mig1 Repressor in Saccharomyces cerevisiae. Mol. Cell. Biol. 18: 6273-6280 [Abstract] [Full Text]  
• Madison, J. M., Dudley, A. M., Winston, F. (1998). Identification and Analysis of Mot3, a Zinc Finger Protein That Binds to the Retrotransposon Ty Long Terminal Repeat (delta ) in Saccharomyces cerevisiae. Mol. Cell. Biol. 18: 1879-1890 [Abstract] [Full Text]  
• Huang, D., Wilson, W. A., Roach, P. J. (1997). Glucose-6-P Control of Glycogen Synthase Phosphorylation in Yeast. J. Biol. Chem. 272: 22495-22501 [Abstract] [Full Text]  
• Cairns, B R, Levinson, R S, Yamamoto, K R, Kornberg, R D (1996). Essential role of Swp73p in the function of yeast Swi/Snf complex.. Genes Dev. 10: 2131-2144 [Abstract]  
• Schmitt, M E, Clayton, D A (1994). Characterization of a unique protein component of yeast RNase MRP: an RNA-binding protein with a zinc-cluster domain.. Genes Dev. 8: 2617-2628 [Abstract]  
• Hirschhorn, J N, Brown, S A, Clark, C D, Winston, F (1992). Evidence that SNF2/SWI2 and SNF5 activate transcription in yeast by altering chromatin structure.. Genes Dev. 6: 2288-2298 [Abstract]  
• Celenza, J., Carlson, M (1986). A yeast gene that is essential for release from glucose repression encodes a protein kinase. Science 233: 1175-1180 [Abstract]  
• Schaus, S. E., Cavalieri, D., Myers, A. G. (2001). Gene transcription analysis of Saccharomyces cerevisiae exposed to neocarzinostatin protein- chromophore complex reveals evidence of DNA damage, a potential mechanism of resistance, and consequences of prolonged exposure. Proc. Natl. Acad. Sci. USA 98: 11075-11080 [Abstract] [Full Text]