This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yocum, R R
Right arrow Articles by Ptashne, M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yocum, R R
Right arrow Articles by Ptashne, M

 Previous Article  |  Next Article 

Mol Cell Biol. 1984 October; 4(10): 1985-1998

Use of lacZ fusions to delimit regulatory elements of the inducible divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.

R R Yocum, S Hanley, R West Jr and M Ptashne

ABSTRACT

We present the DNA sequence of a 914-base pair fragment from Saccharomyces cerevisiae that contains the GAL1-GAL10 divergent promoter, 140 base pairs of GAL10 coding sequence, and 87 base pairs of GAL1 coding sequence. From this fragment, we constructed four pairs of GAL1-lacZ and GAL10-lacZ fusions on various types of yeast plasmid vectors. On each type of vector, the fused genes were induced by galactose and repressed by glucose. The response of a GAL1-lacZ fusion to gal4 and gal80 regulatory mutations was similar to the response of intact chromosomal GAL1 and GAL10 genes. A set of deletions that removed various portions of the GAL10 regulatory sequences from a GAL10-CYC1-lacZ fusion was constructed in vitro. These deletions defined a relatively guanine-cytosine-rich region of 45 base pairs that contained sequences necessary for full-strength galactose induction and an adjacent guanine-cytosine rich 55 base pairs that contained sequences sufficient for weak induction.

Mol Cell Biol. 1984 October; 4(10): 1985-1998




This article has been cited by other articles:

  • Johzuka, K., Horiuchi, T. (2007). RNA polymerase I transcription obstructs condensin association with 35S rRNA coding regions and can cause contraction of long repeat in Saccharomyces cerevisiae. GENES CELLS 12: 759-771 [Abstract] [Full Text]  
  • Li, S., Chen, X., Ruggiero, C., Ding, B., Smerdon, M. J. (2006). Modulation of Rad26- and Rpb9-mediated DNA Repair by Different Promoter Elements. J. Biol. Chem. 281: 36643-36651 [Abstract] [Full Text]  
  • Rohde, J. R., Campbell, S., Zurita-Martinez, S. A., Cutler, N. S., Ashe, M., Cardenas, M. E. (2004). TOR Controls Transcriptional and Translational Programs via Sap-Sit4 Protein Phosphatase Signaling Effectors. Mol. Cell. Biol. 24: 8332-8341 [Abstract] [Full Text]  
  • Krasilnikova, M. M., Mirkin, S. M. (2004). Replication Stalling at Friedreich's Ataxia (GAA)n Repeats In Vivo. Mol. Cell. Biol. 24: 2286-2295 [Abstract] [Full Text]  
  • Kim, J.-H., Polish, J., Johnston, M. (2003). Specificity and Regulation of DNA Binding by the Yeast Glucose Transporter Gene Repressor Rgt1. Mol. Cell. Biol. 23: 5208-5216 [Abstract] [Full Text]  
  • Mosley, A. L., Lakshmanan, J., Aryal, B. K., Ozcan, S. (2003). Glucose-mediated Phosphorylation Converts the Transcription Factor Rgt1 from a Repressor to an Activator. J. Biol. Chem. 278: 10322-10327 [Abstract] [Full Text]  
  • Chang, J. S., Henry, K., Wolf, B. L., Geli, M., Lemmon, S. K. (2002). Protein Phosphatase-1 Binding to Scd5p Is Important for Regulation of Actin Organization and Endocytosis in Yeast. J. Biol. Chem. 277: 48002-48008 [Abstract] [Full Text]  
  • Ozcan, S. (2002). Two Different Signals Regulate Repression and Induction of Gene Expression by Glucose. J. Biol. Chem. 277: 46993-46997 [Abstract] [Full Text]  
  • Hall, D. B., Struhl, K. (2002). The VP16 Activation Domain Interacts with Multiple Transcriptional Components as Determined by Protein-Protein Cross-linking in Vivo. J. Biol. Chem. 277: 46043-46050 [Abstract] [Full Text]  
  • Zhao, C., York, A., Yang, F., Forsthoefel, D. J., Dave, V., Fu, D., Zhang, D., Corado, M. S., Small, S., Seeger, M. A., Ma, J. (2002). The activity of the Drosophila morphogenetic protein Bicoid is inhibited by a domain located outside its homeodomain. Development 129: 1669-1680 [Abstract] [Full Text]  
  • Adam, M., Robert, F., Larochelle, M., Gaudreau, L. (2001). H2A.Z Is Required for Global Chromatin Integrity and for Recruitment of RNA Polymerase II under Specific Conditions. Mol. Cell. Biol. 21: 6270-6279 [Abstract] [Full Text]  
  • Kulish, D., Struhl, K. (2001). TFIIS Enhances Transcriptional Elongation through an Artificial Arrest Site In Vivo. Mol. Cell. Biol. 21: 4162-4168 [Abstract] [Full Text]  
  • Shenhar, G., Kassir, Y. (2001). A Positive Regulator of Mitosis, Sok2, Functions as a Negative Regulator of Meiosis in Saccharomyces cerevisiae. Mol. Cell. Biol. 21: 1603-1612 [Abstract] [Full Text]  
  • Taylor, D. N., Carr, J. P. (2000). The GCD10 subunit of yeast eIF-3 binds the methyltransferase-like domain of the 126 and 183 kDa replicase proteins of tobacco mosaic virus in the yeast two-hybrid system. J. Gen. Virol. 81: 1587-1591 [Abstract] [Full Text]  
  • Leu, J.-Y., Roeder, G. S. (1999). Splicing of the Meiosis-Specific HOP2 Transcript Utilizes a Unique 5' Splice Site. Mol. Cell. Biol. 19: 7933-7943 [Abstract] [Full Text]  
  • Rojo-Niersbach, E., Furukawa, T., Tanese, N. (1999). Genetic Dissection of hTAFII130 Defines a Hydrophobic Surface Required for Interaction with Glutamine-rich Activators. J. Biol. Chem. 274: 33778-33784 [Abstract] [Full Text]  
  • Maggiolini, M., Donze, O., Jeannin, E., Ando, S., Picard, D. (1999). Adrenal Androgens Stimulate the Proliferation of Breast Cancer Cells as Direct Activators of Estrogen Receptor {{alpha}}. Cancer Res. 59: 4864-4869 [Abstract] [Full Text]  
  • Kim, S.-K., Wang, J. C. (1999). Gene silencing via protein-mediated subcellular localization of DNA. Proc. Natl. Acad. Sci. USA 96: 8557-8561 [Abstract] [Full Text]  
  • Bowers, J., Sokolsky, T., Quach, T., Alani, E. (1999). A Mutation in the MSH6 Subunit of the Saccharomyces cerevisiae MSH2-MSH6 Complex Disrupts Mismatch Recognition. J. Biol. Chem. 274: 16115-16125 [Abstract] [Full Text]  
  • Lutfiyya, L. L., Iyer, V. R., DeRisi, J., DeVit, M. J., Brown, P. O., Johnston, M. (1998). Characterization of Three Related Glucose Repressors and Genes They Regulate in Saccharomyces cerevisiae. Genetics 150: 1377-1391 [Abstract] [Full Text]  
  • Louvion, J.-F., Abbas-Terki, T., Picard, D. (1998). Hsp90 Is Required for Pheromone Signaling in Yeast. Mol. Biol. Cell 9: 3071-3083 [Abstract] [Full Text]  
  • Emili, A., Kobayashi, R., Ingles, C. J. (1998). A novel yeast protein influencing the response of RNA polymerase II to transcriptional activators. Proc. Natl. Acad. Sci. USA 95: 11122-11127 [Abstract] [Full Text]  
  • Kahana, J. A., Schlenstedt, G., Evanchuk, D. M., Geiser, J. R., Hoyt, M. A., Silver, P. A. (1998). The Yeast Dynactin Complex Is Involved in Partitioning the Mitotic Spindle between Mother and Daughter Cells during Anaphase B. Mol. Biol. Cell 9: 1741-1756 [Abstract] [Full Text]  
  • Zheng, W., Johnston, S. A. (1998). The Nucleic Acid Binding Activity of Bleomycin Hydrolase Is Involved in Bleomycin Detoxification. Mol. Cell. Biol. 18: 3580-3585 [Abstract] [Full Text]  
  • Ramaswamy, N. T., Li, L., Khalil, M., Cannon, J. F. (1998). Regulation of Yeast Glycogen Metabolism and Sporulation by Glc7p Protein Phosphatase. Genetics 149: 57-72 [Abstract] [Full Text]  
  • Kuchin, S., Carlson, M. (1998). Functional Relationships of Srb10-Srb11 Kinase, Carboxy-Terminal Domain Kinase CTDK-I, and Transcriptional Corepressor Ssn6-Tup1. Mol. Cell. Biol. 18: 1163-1171 [Abstract] [Full Text]  
  • Otterson, G. A., Chen, W.-d., Coxon, A. B., Khleif, S. N., Kaye, F. J. (1997). Incomplete penetrance of familial retinoblastoma linked to germ-line mutations that result in partial loss of RB function. Proc. Natl. Acad. Sci. USA 94: 12036-12040 [Abstract] [Full Text]  
  • Meng, J.-J., Bornslaeger, E. A., Green, K. J., Steinert, P. M., Ip, W. (1997). Two-hybrid Analysis Reveals Fundamental Differences in Direct Interactions between Desmoplakin and Cell Type-specific Intermediate Filaments. J. Biol. Chem. 272: 21495-21503 [Abstract] [Full Text]  
  • Graden, J. A., Winge, D. R. (1997). Copper-mediated repression of the activation domain in the yeast Mac1p transcription factor. Proc. Natl. Acad. Sci. USA 94: 5550-5555 [Abstract] [Full Text]  
  • Russell, S. J., Sathyanarayana, U. G., Johnston, S. A. (1996). Isolation and Characterization of SUG2. A NOVEL ATPase FAMILY COMPONENT OF THE YEAST 26S PROTEASOME. J. Biol. Chem. 271: 32810-32817 [Abstract] [Full Text]  
  • Louvion, J.-F., Warth, R., Picard, D. (1996). Two eukaryote-specific regions of Hsp82 are dispensable for its viability and signal transduction functions in yeast. Proc. Natl. Acad. Sci. USA 93: 13937-13942 [Abstract] [Full Text]  
  • Paull, T T, Carey, M, Johnson, R C (1996). Yeast HMG proteins NHP6A/B potentiate promoter-specific transcriptional activation in vivo and assembly of preinitiation complexes in vitro.. Genes Dev. 10: 2769-2781 [Abstract]  
  • Craig, A. W.B., Cosentino, G. P., Donze, O., Sonenberg, N. (1996). The Kinase Insert Domain of Interferon-induced Protein Kinase PKR Is Required for Activity but Not for Interaction with the Pseudosubstrate K3L. J. Biol. Chem. 271: 24526-24533 [Abstract] [Full Text]  
  • Lee, M S, Henry, M, Silver, P A (1996). A protein that shuttles between the nucleus and the cytoplasm is an important mediator of RNA export.. Genes Dev. 10: 1233-1246 [Abstract]  
  • Meng, J.-j., Khan, S., Ip, W. (1996). Intermediate Filament Protein Domain Interactions as Revealed by Two-hybrid Screens. J. Biol. Chem. 271: 1599-1604 [Abstract] [Full Text]  
  • Ma, X, Yuan, D, Diepold, K, Scarborough, T, Ma, J (1996). The Drosophila morphogenetic protein Bicoid binds DNA cooperatively. Development 122: 1195-1206 [Abstract]  
  • Schena, M, Lloyd, A M, Davis, R W (1993). The HAT4 gene of Arabidopsis encodes a developmental regulator.. Genes Dev. 7: 367-379 [Abstract]  
  • Yoshinaga, S., Peterson, C., Herskowitz, I, Yamamoto, K. (1992). Roles of SWI1, SWI2, and SWI3 proteins for transcriptional enhancement by steroid receptors. Science 258: 1598-1604 [Abstract]  
  • Schena, M, Freedman, L P, Yamamoto, K R (1989). Mutations in the glucocorticoid receptor zinc finger region that distinguish interdigitated DNA binding and transcriptional enhancement activities.. Genes Dev. 3: 1590-1601 [Abstract]  
  • Duronio, R., Towler, D., Heuckeroth, R., Gordon, J. (1989). Disruption of the yeast N-myristoyl transferase gene causes recessive lethality. Science 243: 796-800 [Abstract]  
  • Schena, M, Yamamoto, K. (1988). Mammalian glucocorticoid receptor derivatives enhance transcription in yeast. Science 241: 965-967 [Abstract]  
  • West, R W, Chen, S M, Putz, H, Butler, G, Banerjee, M (1987). GAL1-GAL10 divergent promoter region of Saccharomyces cerevisiae contains negative control elements in addition to functionally separate and possibly overlapping upstream activating sequences.. Genes Dev. 1: 1118-1131 [Abstract]  
  • Keegan, L, Gill, G, Ptashne, M (1986). Separation of DNA binding from the transcription-activating function of a eukaryotic regulatory protein. Science 231: 699-704 [Abstract]  
  • Pillai, B., Sampath, V., Sharma, N., Sadhale, P. (2001). Rpb4, a Non-essential Subunit of Core RNA Polymerase II of Saccharomyces cerevisiae Is Important for Activated Transcription of a Subset of Genes. J. Biol. Chem. 276: 30641-30647 [Abstract] [Full Text]  
  • Kajkowski, E. M., Lo, C. F., Ning, X., Walker, S., Sofia, H. J., Wang, W., Edris, W., Chanda, P., Wagner, E., Vile, S., Ryan, K., McHendry-Rinde, B., Smith, S. C., Wood, A., Rhodes, K. J., Kennedy, J. D., Bard, J., Jacobsen, J. S., Ozenberger, B. A. (2001). beta -Amyloid Peptide-induced Apoptosis Regulated by a Novel Protein Containing a G Protein Activation Module. J. Biol. Chem. 276: 18748-18756 [Abstract] [Full Text]  
  • Kosugi, S., Ohashi, Y. (2002). Interaction of the Arabidopsis E2F and DP Proteins Confers Their Concomitant Nuclear Translocation and Transactivation. Plant Physiol. 128: 833-843 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»