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Mol Cell Biol. 1984 October; 4(10): 2227-2230

Error-prone mutagenesis detected in mammalian cells by a shuttle vector containing the supF gene of Escherichia coli.

ABSTRACT

When a shuttle vector containing a tyrosine suppressor tRNA (supF) gene as a target for mutagenesis replicated in a monkey kidney cell line, the frequency of SupF+ mutations was 2.3 +/- 0.5 x 10(-3). When the host cells were treated with ethyl methanesulfonate 40 h before transfection, a 10-fold increase in SupF+ mutation frequency was observed. These results supported the hypothesis that a damage-inducible mutagenic pathway exists in mammalian cells and also demonstrated the utility of this shuttle vector for the study of mutagenesis in mammalian cells.

This article has been cited by other articles:

• Lewis, P.D., Harvey, J.S., Waters, E.M., Skibinski, D.O.F., Parry, J.M. (2001). Spontaneous mutation spectra in supF: comparative analysis of mammalian cell line base substitution spectra. Mutagenesis 16: 503-515 [Abstract] [Full Text]