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Mol Cell Biol. 1984 November; 4(11): 2486-2497

Proteins encoded by the human c-myc oncogene: differential expression in neoplastic cells.

S R Hann and R N Eisenman

ABSTRACT

To examine myc protein products in the wide variety of human tumor cells having alterations of the c-myc locus, we have prepared an antiserum against a synthetic peptide corresponding to the predicted C-terminal sequence of the human c-myc protein. This antiserum (anti-hu-myc 12C) specifically precipitated two proteins of 64 and 67 kilodaltons in quantities ranging from low levels in normal fibroblasts to 10-fold-higher levels in Epstein-Barr virus-immortalized and Burkitt's lymphoma cell lines, to 20- to 60-fold-higher levels in cell lines having amplified c-myc. The p64 and p67 proteins were found to be highly related by partial V8 proteolytic mapping, and both were demonstrated to be encoded by the c-myc oncogene, using hybrid-selected translation of myc-specific RNA. In addition, the p64 protein was specifically precipitated from cells transfected with a translocated c-myc gene. Both p64 and p67 were found to be nuclear phosphoproteins with extremely short half-lives. In tumor cell lines having alterations at the c-myc locus due to amplification or translocation, we observed a significant change in the expression of p64 relative to p67 when compared with normal or Epstein-Bar virus-immortalized cells.

Mol Cell Biol. 1984 November; 4(11): 2486-2497




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  • Sun, Y., Clark, E. A. (1999). Expression of the c-myc Proto-oncogene Is Essential for HIV-1 Infection in Activated T Cells. JEM 189: 1391-1398 [Abstract] [Full Text]  
  • Ruf, I. K., Rhyne, P. W., Yang, H., Borza, C. M., Hutt-Fletcher, L. M., Cleveland, J. L., Sample, J. T. (1999). Epstein-Barr Virus Regulates c-MYC, Apoptosis, and Tumorigenicity in Burkitt Lymphoma. Mol. Cell. Biol. 19: 1651-1660 [Abstract] [Full Text]  
  • Aziz, N., Cherwinski, H., McMahon, M. (1999). Complementation of Defective Colony-Stimulating Factor 1 Receptor Signaling and Mitogenesis by Raf and v-Src. Mol. Cell. Biol. 19: 1101-1115 [Abstract] [Full Text]  
  • Flinn, E. M., Busch, C. M. C., Wright, A. P. H. (1998). myc Boxes, Which Are Conserved in myc Family Proteins, Are Signals for Protein Degradation via the Proteasome. Mol. Cell. Biol. 18: 5961-5969 [Abstract] [Full Text]  
  • Facchini, L., Penn, L. Z. (1998). The molecular role of Myc in growth and transformation: recent discoveries lead to new insights. FASEB J. 12: 633-651 [Abstract] [Full Text]  
  • Zamorano, J., Wang, H. Y., Wang, R., Shi, Y., Longmore, G. D., Keegan, A. D. (1998). Regulation of Cell Growth by IL-2: Role of STAT5 in Protection from Apoptosis But Not in Cell Cycle Progression. J. Immunol. 160: 3502-3512 [Abstract] [Full Text]  
  • Hurlin, P J, Queva, C, Eisenman, R N (1997). Mnt, a novel Max-interacting protein is coexpressed with Myc in proliferating cells and mediates repression at Myc binding sites.. Genes Dev. 11: 44-58 [Abstract]  
  • Tikhonenko, A. T., Black, D. J., Linial, M. L. (1996). Viral Myc Oncoproteins in Infected Fibroblasts Down-modulate Thrombospondin-1, a Possible Tumor Suppressor Gene. J. Biol. Chem. 271: 30741-30747 [Abstract] [Full Text]  
  • Kaptein, J. S., Lin, C.-K. E., Wang, C. L., Nguyen, T. T., Kalunta, C. I., Park, E., Chen, F.-S., Lad, P. M. (1996). Anti-IgM-mediated Regulation of c-myc and Its Possible Relationship to Apoptosis. J. Biol. Chem. 271: 18875-18884 [Abstract] [Full Text]  
  • Higashide, T., Murakami, A., McLaren, M. J., Inana, G. (1996). Cloning of the cDNA for a Novel Photoreceptor Protein. J. Biol. Chem. 271: 1797-1804 [Abstract] [Full Text]  
  • Hann, S R, Dixit, M, Sears, R C, Sealy, L (1994). The alternatively initiated c-Myc proteins differentially regulate transcription through a noncanonical DNA-binding site.. Genes Dev. 8: 2441-2452 [Abstract]  
  • Hann, S R, Sloan-Brown, K, Spotts, G D (1992). Translational activation of the non-AUG-initiated c-myc 1 protein at high cell densities due to methionine deprivation.. Genes Dev. 6: 1229-1240 [Abstract]  
  • Berberich, S J, Cole, M D (1992). Casein kinase II inhibits the DNA-binding activity of Max homodimers but not Myc/Max heterodimers.. Genes Dev. 6: 166-176 [Abstract]  
  • Harris, L., Talian, J., Zelenka, P. (1992). Contrasting patterns of c-myc and N-myc expression in proliferating, quiescent, and differentiating cells of the embryonic chicken lens. Development 115: 813-820 [Abstract]  
  • Blackwood, E M, Luscher, B, Eisenman, R N (1992). Myc and Max associate in vivo.. Genes Dev. 6: 71-80 [Abstract]  
  • Blackwood, E., Eisenman, R. (1991). Max: a helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex with Myc. Science 251: 1211-1217 [Abstract]  
  • Blackwood, E.M., Luscher, B., Kretzner, L., Eisenman, R.N. (1991). The Myc:Max Protein Complex and Cell Growth Regulation. Cold Spring Harb Symp Quant Biol 56: 109-117 [Abstract]  
  • Blackwell, T., Kretzner, L, Blackwood, E., Eisenman, R., Weintraub, H (1990). Sequence-specific DNA binding by the c-Myc protein. Science 250: 1149-1151 [Abstract]  
  • Littlewood, T.D., Evan, G.I. (1990). The Role of myc Oncogenes in Cell Growth and Differentiation. ADR 4: 69-79 [Abstract]  
  • Krebs, E.G., Eisenman, R.N., Kijenzel, E.A., Litchfield, D.W., Lozeman, F.J., Luscher, B., Sommercorn, J. (1988). Casein Kinase II as a Potentially Important Enzyme Concerned with Signal Transduction. Cold Spring Harb Symp Quant Biol 53: 77-84 [Abstract]  
  • DePinho, R A, Hatton, K S, Tesfaye, A, Yancopoulos, G D, Alt, F W (1987). The human myc gene family: structure and activity of L-myc and an L-myc pseudogene.. Genes Dev. 1: 1311-1326 [Abstract]  
  • Cesarman, E, Dalla-Favera, R, Bentley, D, Groudine, M (1987). Mutations in the first exon are associated with altered transcription of c-myc in Burkitt lymphoma. Science 238: 1272-1275 [Abstract]  
  • Hay, N, Bishop, J M, Levens, D (1987). Regulatory elements that modulate expression of human c-myc.. Genes Dev. 1: 659-671 [Abstract]  
  • Bachmair, A, Finley, D, Varshavsky, A (1986). In vivo half-life of a protein is a function of its amino-terminal residue. Science 234: 179-186 [Abstract]  
  • Slamon, D., Boone, T., Seeger, R., Keith, D., Chazin, V, Lee, H., Souza, L. (1986). Identification and characterization of the protein encoded by the human N-myc oncogene. Science 232: 768-772 [Abstract]  
  • Linial, M, Gunderson, N, Groudine, M (1985). Enhanced transcription of c-myc in bursal lymphoma cells requires continuous protein synthesis. Science 230: 1126-1132 [Abstract]  
  • Androphy, E., Schiller, J., Lowy, D. (1985). Identification of the protein encoded by the E6 transforming gene of bovine papillomavirus. Science 230: 442-445 [Abstract]  
  • Law, B. K., Waltner-Law, M. E., Entingh, A. J., Chytil, A., Aakre, M. E., Norgaard, P., Moses, H. L. (2000). Salicylate-induced Growth Arrest Is Associated with Inhibition of p70s6k and Down-regulation of c-Myc, Cyclin D1, Cyclin A, and Proliferating Cell Nuclear Antigen. J. Biol. Chem. 275: 38261-38267 [Abstract] [Full Text]  


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