This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Innis, J W Articles by Scott, W A Search for Related Content PubMed PubMed Citation Articles by Innis, J W Articles by Scott, W A

 Previous Article  |  Next Article 

Mol Cell Biol. 1984 August; 4(8): 1499-1507

DNA replication and chromatin structure of simian virus 40 insertion mutants.

ABSTRACT

Insertion of DNA segments into the nuclease-sensitive region of simian virus 40 alters both replication efficiency and chromatin structure. Mutants containing large insertions between the simian virus 40 origin of replication (ori site) and the 21-base-pair repeated sequences replicated poorly when assayed by transfection into COS-1 cells. Replication of mutants with shorter insertions was moderately reduced. This effect was cis-acting and independent of the nucleotide sequence of the insert. The nuclease-sensitive chromatin structure was retained in these mutants, but the pattern of cleavage sites was displaced in the late direction from the ori site. New cleavage sites appeared within the inserted sequences, suggesting that information specifying the nuclease-sensitive chromatin structure is located on the late side of the inserts. Accessibility to BglI (which cleaves within the ori site) was reduced in the larger insertion mutants. These results support the conclusion that efficient function of the viral origin of replication is correlated with its proximity to an altered chromatin structure.

This article has been cited by other articles:

• Wilderman, P. J., Hu, B., Woodworth, M. E. (1999). Conformational Changes in Simian Virus 40 Rearranged Regulatory Regions: Effects of the 21-Base-Pair Promoters and Their Location. J. Virol. 73: 10254-10263 [Abstract] [Full Text]  
• Friez, M., Hermansen, R., Milavetz, B. (1999). Chromatin Structure of the Simian Virus 40 Late Promoter: a Deletional Analysis. J. Virol. 73: 1990-1997 [Abstract] [Full Text]  
• Nguyen-Huynh, A. T., Schaffer, P. A. (1998). Cellular Transcription Factors Enhance Herpes Simplex Virus Type 1 oriS-Dependent DNA Replication. J. Virol. 72: 3635-3645 [Abstract] [Full Text]