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Mol Cell Biol. 1984 September; 4(9): 1929-1934

Posttranscriptional modulation of gene expression in cultured rat hepatocytes.

D M Jefferson, D F Clayton, J E Darnell Jr and L M Reid

ABSTRACT

The maintenance of high levels of two liver-specific mRNAs in cultured hepatocytes was achieved in a serum-free hormonally defined cell culture medium. However, this maintenance of liver-specific mRNA levels did not correlate with the level of transcription of the genes but was apparently due to increased stabilization of the tissue-specific mRNAs. The mRNA stabilization did not occur in serum-supplemented medium. In both defined and serum-supplemented medium, actin and tubulin mRNAs were also greatly increased, in both cases predominantly if not entirely due to increased mRNA stability.

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Mol Cell Biol. 1984 September; 4(9): 1929-1934

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