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Mol Cell Biol. 1985 January; 5(1): 93-98

Elimination of micronuclear specific DNA sequences early in anlagen development.

ABSTRACT

After conjugation in Tetrahymena thermophila, the old macronuclei degenerate, and new macronuclei (anlagen) develop. During anlagen development a number of DNA sequences found in the micronuclear genome (micronuclear limited sequences) are eliminated from the anlagen. A cloned copy of a repetitive micronuclear limited sequence has been used to determine the developmental stage at which micronuclear limited sequences are eliminated. DNAs from anlagen of various developmental stages were examined by Southern analysis. It was found that micronuclear limited sequences are present in 4C anlagen and essentially absent in 8C and 16C anlagen. The precipitous loss of these sequences in the 8C anlagen rules out under-replication as the mechanism for the loss and suggests that these sequences are specifically degraded early during anlagen development.

This article has been cited by other articles:

• Yakisich, J. S., Kapler, G. M. (2006). Deletion of the Tetrahymena thermophila rDNA replication fork barrier region disrupts macronuclear rDNA excision and creates a fragile site in the micronuclear genome. Nucleic Acids Res 34: 620-634 [Abstract] [Full Text]  
• Wuitschick, J. D., Karrer, K. M. (2003). Diverse Sequences within Tlr Elements Target Programmed DNA Elimination in Tetrahymena thermophila. Eukaryot Cell 2: 678-689 [Abstract] [Full Text]