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Mol Cell Biol. 1985 December; 5(12): 3403-3409

Vaccinia virus expression vector: coexpression of beta-galactosidase provides visual screening of recombinant virus plaques.

ABSTRACT

We constructed a plasmid coexpression vector that directs the insertion of a foreign gene of interest together with the Escherichia coli beta-galactosidase (beta gal) gene into the thymidine kinase (TK) locus of the vaccinia virus genome. Tissue culture cells that had been infected with vaccinia virus were transfected with a plasmid vector containing a foreign gene. TK- recombinants could be selected by a plaque assay on TK- cells in the presence of 5-bromodeoxyuridine and distinguished from spontaneous TK- mutants by the addition of a beta-gal indicator to the agarose overlay. Plaques that expressed beta-gal stained dark blue within several hours at 37 degrees C. Alternatively, TK- selection could be eliminated, and recombinant plaques could be readily identified solely by their blue color. The reverse procedure, in which the starting virus expresses beta-gal (i.e., forms blue plaques) and the desired recombinant has deleted the entire beta-gal gene (i.e., forms white plaques), is another alternative. Each protocol was tested by constructing vaccinia virus recombinants that express hepatitis B virus surface antigen.

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• Marais,, D., Passmore,, J.-A., Maclean,, J., Rose, R., Williamson, A.-L. (1999). A recombinant human papillomavirus (HPV) type 16 L1-vaccinia virus murine challenge model demonstrates cell-mediated immunity against HPV virus-like particles. J. Gen. Virol. 80: 2471-2475 [Abstract] [Full Text]  
• Montoya, M., Del Val, M. (1999). Intracellular Rate-Limiting Steps in MHC Class I Antigen Processing. J. Immunol. 163: 1914-1922 [Abstract] [Full Text]  
• Kalams, S. A., Goulder, P. J., Shea, A. K., Jones, N. G., Trocha, A. K., Ogg, G. S., Walker, B. D. (1999). Levels of Human Immunodeficiency Virus Type 1-Specific Cytotoxic T-Lymphocyte Effector and Memory Responses Decline after Suppression of Viremia with Highly Active Antiretroviral Therapy. J. Virol. 73: 6721-6728 [Abstract] [Full Text]  
• Anton, L. C., Schubert, U., Bacik, I., Princiotta, M. F., Wearsch, P. A., Gibbs, J., Day, P. M., Realini, C., Rechsteiner, M. C., Bennink, J. R., Yewdell, J. W. (1999). Intracellular Localization of Proteasomal Degradation of a Viral Antigen. J. Cell Biol. 146: 113-124 [Abstract] [Full Text]  
• Osman, M., Kubo, T., Gill, J., Neipel, F., Becker, M., Smith, G., Weiss, R., Gazzard, B., Boshoff, C., Gotch, F. (1999). Identification of Human Herpesvirus 8-Specific Cytotoxic T-Cell Responses. J. Virol. 73: 6136-6140 [Abstract] [Full Text]  
• Matsui, S., Ahlers, J. D., Vortmeyer, A. O., Terabe, M., Tsukui, T., Carbone, D. P., Liotta, L. A., Berzofsky, J. A. (1999). A Model for CD8+ CTL Tumor Immunosurveillance and Regulation of Tumor Escape by CD4 T Cells Through an Effect on Quality of CTL. J. Immunol. 163: 184-193 [Abstract] [Full Text]  
• Holzer, G. W., Remp, G., Antoine, G., Pfleiderer, M., Enzersberger, O. M., Emsenhuber, W., Hämmerle, T., Gruber, F., Urban, C., Falkner, F. G., Dorner, F. (1999). Highly Efficient Induction of Protective Immunity by a Vaccinia Virus Vector Defective in Late Gene Expression. J. Virol. 73: 4536-4542 [Abstract] [Full Text]  
• Belyakov, I. M., Moss, B., Strober, W., Berzofsky, J. A. (1999). Mucosal vaccination overcomes the barrier to recombinant vaccinia immunization caused by preexisting poxvirus immunity. Proc. Natl. Acad. Sci. USA 96: 4512-4517 [Abstract] [Full Text]  
• Zhang, H., Hanecak, R., Brown-Driver, V., Azad, R., Conklin, B., Fox, M. C., Anderson, K. P. (1999). Antisense Oligonucleotide Inhibition of Hepatitis C Virus (HCV) Gene Expression in Livers of Mice Infected with an HCV-Vaccinia Virus Recombinant. Antimicrob. Agents Chemother. 43: 347-353 [Abstract] [Full Text]  
• Robert-Guroff, M., Kaur, H., Patterson, L. J., Leno, M., Conley, A. J., McKenna, P. M., Markham, P. D., Richardson, E., Aldrich, K., Arora, K., Murty, L., Carter, L., Zolla-Pazner, S., Sinangil, F. (1998). Vaccine Protection against a Heterologous, Non-Syncytium-Inducing, Primary Human Immunodeficiency Virus. J. Virol. 72: 10275-10280 [Abstract] [Full Text]  
• Bronte, V., Wang, M., Overwijk, W. W., Surman, D. R., Pericle, F., Rosenberg, S. A., Restifo, N. P. (1998). Apoptotic Death of CD8+ T Lymphocytes After Immunization: Induction of a Suppressive Population of Mac-1+/Gr-1+ Cells. J. Immunol. 161: 5313-5320 [Abstract] [Full Text]  
• Henke, A., Wagner, E., Whitton, J. L., Zell, R., Stelzner, A. (1998). Protection of Mice against Lethal Coxsackievirus B3 Infection by Using DNA Immunization. J. Virol. 72: 8327-8331 [Abstract] [Full Text]  
• Bembridge, G. P., Garcia-Beato, R., Lopez, J. A., Melero, J. A., Taylor, G. (1998). Subcellular Site of Expression and Route of Vaccination Influence Pulmonary Eosinophilia Following Respiratory Syncytial Virus Challenge in BALB/c Mice Sensitized to the Attachment G Protein. J. Immunol. 161: 2473-2480 [Abstract] [Full Text]  
• Wessely, R., Henke, A., Zell, R., Kandolf, R., Knowlton, K. U. (1998). Low-Level Expression of a Mutant Coxsackieviral cDNA Induces a Myocytopathic Effect in Culture : An Approach to the Study of Enteroviral Persistence in Cardiac Myocytes. Circulation 98: 450-457 [Abstract] [Full Text]  
• Billadeau, D. D., Brumbaugh, K. M., Dick, C. J., Schoon, R. A., Bustelo, X. R., Leibson, P. J. (1998). The Vav-Rac1 Pathway in Cytotoxic Lymphocytes Regulates the Generation of Cell-mediated Killing. J. Exp. Med. 188: 549-559 [Abstract] [Full Text]  
• Yamanaka, M. K., Yilma, T. (1998). Altered Plaque Formation by Recombinant Vaccinia Virus Expressing Simian Immunodeficiency Virus Nef. J. Virol. 72: 5291-5295 [Abstract] [Full Text]  
• Johnson, T. R., Johnson, J. E., Roberts, S. R., Wertz, G. W., Parker, R. A., Graham, B. S. (1998). Priming with Secreted Glycoprotein G of Respiratory Syncytial Virus (RSV) Augments Interleukin-5 Production and Tissue Eosinophilia after RSV Challenge. J. Virol. 72: 2871-2880 [Abstract] [Full Text]  
• Jooss, K., Ertl, H. C. J., Wilson, J. M. (1998). Cytotoxic T-Lymphocyte Target Proteins and Their Major Histocompatibility Complex Class I Restriction in Response to Adenovirus Vectors Delivered to Mouse Liver. J. Virol. 72: 2945-2954 [Abstract] [Full Text]  
• Kulig, K., Nandi, D., Bacik, I., Monaco, J. J., Vukmanovic, S. (1998). Physical and Functional Association of the Major Histocompatibility Complex Class I Heavy Chain alpha 3 Domain with the Transporter Associated with Antigen Processing. J. Exp. Med. 187: 865-874 [Abstract] [Full Text]