This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Subramani, S Articles by Rubnitz, J Search for Related Content PubMed PubMed Citation Articles by Subramani, S Articles by Rubnitz, J

 Previous Article  |  Next Article 

Mol Cell Biol. 1985 April; 5(4): 659-666

Recombination events after transient infection and stable integration of DNA into mouse cells.

ABSTRACT

To investigate the recombinational machinery of mammalian cells, we have constructed plasmids that can be used as substrates for homologous recombination. These plasmids contain two truncated nontandem, but overlapping, segments of the neomycin resistance gene, separated by the transcription unit for the xanthine guanine phosphoribosyl transferase gene. Recombination between the two nonfunctional neomycin gene sequences generates an intact neomycin resistance gene that is functional in both bacteria and mammalian cells. Using these plasmid substrates, we have characterized the frequencies and products of recombination events that occur in mouse 3T6 cells soon after transfection and also after stable integration of these DNAs. Among the chromosomal recombination events, we have characterized apparent deletion events that can be accounted for by intrachromatid recombination or unequal sister chromatid exchanges. Other recombination events like chromosomal inversions and possible gene conversion events in an amplification unit are also described.

This article has been cited by other articles:

• Read, L. R., Raynard, S. J., Ruksc, A., Baker, M. D. (2004). Gene repeat expansion and contraction by spontaneous intrachromosomal homologous recombination in mammalian cells. Nucleic Acids Res 32: 1184-1196 [Abstract] [Full Text]  
• Avkin, S., Adar, S., Blander, G., Livneh, Z. (2002). Quantitative measurement of translesion replication in human cells: Evidence for bypass of abasic sites by a replicative DNA polymerase. Proc. Natl. Acad. Sci. USA 99: 3764-3769 [Abstract] [Full Text]  
• Raynard, S. J., Read, L. R., Baker, M. D. (2002). Evidence for the Murine IgH {micro} Locus Acting as a Hot Spot for Intrachromosomal Homologous Recombination. J. Immunol. 168: 2332-2339 [Abstract] [Full Text]  
• Inoue, N., Russell, D. W. (1998). Packaging Cells Based on Inducible Gene Amplification for the Production of Adeno-Associated Virus Vectors. J. Virol. 72: 7024-7031 [Abstract] [Full Text]  
• Carlson, L M, McCormack, W T, Postema, C E, Humphries, E H, Thompson, C B (1990). Templated insertions in the rearranged chicken IgL V gene segment arise by intrachromosomal gene conversion.. Genes Dev. 4: 536-547 [Abstract]