This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Learned, R M
Right arrow Articles by Tjian, R
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Learned, R M
Right arrow Articles by Tjian, R

 Previous Article  |  Next Article 

Mol Cell Biol. 1985 June; 5(6): 1358-1369

Purification and characterization of a transcription factor that confers promoter specificity to human RNA polymerase I.

R M Learned, S Cordes and R Tjian

ABSTRACT

A whole-cell HeLa extract was fractionated into two components required for accurate in vitro transcription of human rRNA. One fraction contained endogenous RNA polymerase I, and the second component contained a factor (SL1) that confers promoter selectivity to RNA polymerase I. Analysis of mutant templates suggests that the core control element of the rRNA promoter is required for activation of transcription by SL1. We purified SL1 approximately 100,000-fold by column chromatography and have shown that the addition of SL1 can reprogram the otherwise nonpermissive mouse transcription system to recognize and initiate accurate RNA synthesis from human rDNA. Antibodies raised against SL1 bind preferentially to a protein localized in the nucleolus of primate cells and specifically inhibit in vitro transcription initiating from the human rRNA promoter. By contrast, anti-SL1 does not react with the nucleolus of rodent cells and has no effect on the in vitro synthesis of mouse rRNA by a transcription system derived from mouse cells. These findings suggest that SL1 is a selectivity factor present in the nucleolus that imparts promoter recognition to RNA polymerase I and that can discriminate between rRNA promoters from different species.

Mol Cell Biol. 1985 June; 5(6): 1358-1369




This article has been cited by other articles:

  • Friedrich, J. K., Panov, K. I., Cabart, P., Russell, J., Zomerdijk, J. C. B. M. (2005). TBP-TAF Complex SL1 Directs RNA Polymerase I Pre-initiation Complex Formation and Stabilizes Upstream Binding Factor at the rDNA Promoter. J. Biol. Chem. 280: 29551-29558 [Abstract] [Full Text]  
  • Chen, D., Belmont, A. S., Huang, S. (2004). Upstream binding factor association induces large-scale chromatin decondensation. Proc. Natl. Acad. Sci. USA 101: 15106-15111 [Abstract] [Full Text]  
  • Grummt, I. (2003). Life on a planet of its own: regulation of RNA polymerase I transcription in the nucleolus. Genes Dev. 17: 1691-1702 [Full Text]  
  • Hirschler-Laszkiewicz, I., Cavanaugh, A. H., Mirza, A., Lun, M., Hu, Q., Smink, T., Rothblum, L. I. (2003). Rrn3 Becomes Inactivated in the Process of Ribosomal DNA Transcription. J. Biol. Chem. 278: 18953-18959 [Abstract] [Full Text]  
  • Al-Khouri, A. M., Paule, M. R. (2002). A Novel RNA Polymerase I Transcription Initiation Factor, TIF-IE, Commits rRNA Genes by Interaction with TIF-IB, Not by DNA Binding. Mol. Cell. Biol. 22: 750-761 [Abstract] [Full Text]  
  • Panov, K. I., Friedrich, J. K., Zomerdijk, J. C. B. M. (2001). A Step Subsequent to Preinitiation Complex Assembly at the Ribosomal RNA Gene Promoter Is Rate Limiting for Human RNA Polymerase I-Dependent Transcription. Mol. Cell. Biol. 21: 2641-2649 [Abstract] [Full Text]  
  • Aprikian, P., Moorefield, B., Reeder, R. H. (2000). TATA Binding Protein Can Stimulate Core-Directed Transcription by Yeast RNA Polymerase I. Mol. Cell. Biol. 20: 5269-5275 [Abstract] [Full Text]  
  • Balajee, A. S., Machwe, A., May, A., Gray, M. D., Oshima, J., Martin, G. M., Nehlin, J. O., Brosh, R., Orren, D. K., Bohr, V. A. (1999). The Werner Syndrome Protein Is Involved in RNA Polymerase II Transcription. Mol. Biol. Cell 10: 2655-2668 [Abstract] [Full Text]  
  • Frieman, M., Chen, Z. J., Saez-Vasquez, J., Shen, L. A., Pikaard, C. S. (1999). RNA Polymerase I Transcription in a Brassica Interspecific Hybrid and Its Progenitors: Tests of Transcription Factor Involvement in Nucleolar Dominance. Genetics 152: 451-460 [Abstract] [Full Text]  
  • Tuan, J. C., Zhai, W., Comai, L. (1999). Recruitment of TATA-Binding Protein-TAFI Complex SL1 to the Human Ribosomal DNA Promoter Is Mediated by the Carboxy-Terminal Activation Domain of Upstream Binding Factor (UBF) and Is Regulated by UBF Phosphorylation. Mol. Cell. Biol. 19: 2872-2879 [Abstract] [Full Text]  
  • Alzuherri, H. M., White, R. J. (1999). Regulation of RNA Polymerase I Transcription in Response to F9 Embryonal Carcinoma Stem Cell Differentiation. J. Biol. Chem. 274: 4328-4334 [Abstract] [Full Text]  
  • Cavender, J. F., Mummert, C., Tevethia, M. J. (1999). Transactivation of a Ribosomal Gene by Simian Virus 40 Large-T Antigen Requires at Least Three Activities of the Protein. J. Virol. 73: 214-224 [Abstract] [Full Text]  
  • Hannan, K. M., Rothblum, L. I., Jefferson, L. S. (1998). Regulation of ribosomal DNA transcription by insulin. Am. J. Physiol. Cell Physiol. 275: C130-C138 [Abstract] [Full Text]  
  • Lee, T. I., Young, R. A. (1998). Regulation of gene expression by TBP-associated proteins. Genes Dev. 12: 1398-1408 [Full Text]  
  • Zhai, W, Tuan, J A, Comai, L (1997). SV40 large T antigen binds to the TBP-TAF(I) complex SL1 and coactivates ribosomal RNA transcription.. Genes Dev. 11: 1605-1617 [Abstract]  
  • Heix, J., Zomerdijk, J. C. B. M., Ravanpay, A., Tjian, R., Grummt, I. (1997). Cloning of murine RNA polymerase I-specific TAF factors: Conserved interactions between the subunits of the species-specific transcription initiation factor TIF-IB/SL1. Proc. Natl. Acad. Sci. USA 94: 1733-1738 [Abstract] [Full Text]  
  • Bell, P, Mais, C, McStay, B, Scheer, U (1997). Association of the nucleolar transcription factor UBF with the transcriptionally inactive rRNA genes of pronuclei and early Xenopus embryos. J. Cell Sci. 110: 2053-2063 [Abstract]  
  • Datta, P. K., Ghosh, A. K., Jacob, S. T. (1995). The RNA Polymerase I Promoter-activating Factor CPBF Is Functionally and Immunologically Related to the Basic Helix-Loop-Helix-Zipper DNA-binding Protein USF. J. Biol. Chem. 270: 8637-8641 [Abstract] [Full Text]  
  • Comai, L, Zomerdijk, J., Beckmann, H, Zhou, S, Admon, A, Tjian, R (1994). Reconstitution of transcription factor SL1: exclusive binding of TBP by SL1 or TFIID subunits. Science 266: 1966-1972 [Abstract]  
  • Zomerdijk, J., Beckmann, H, Comai, L, Tjian, R (1994). Assembly of transcriptionally active RNA polymerase I initiation factor SL1 from recombinant subunits. Science 266: 2015-2018 [Abstract]  
  • Hernandez, N (1993). TBP, a universal eukaryotic transcription factor?. Genes Dev. 7: 1291-1308  
  • Tanese, N., Tjian, R. (1993). Coactivators and TAFs: A New Class of Eukaryotic Transcription Factors That Connect Activators to the Basal Machinery. Cold Spring Harb Symp Quant Biol 58: 179-185 [Abstract]  
  • Rodrigo, R. M., Rendon, M. C., Torreblanca, J., Garcia-Herdugo, G., Moreno, F. J. (1992). Characterization and immunolocalization of RNA polymerase I transcription factor UBF with anti-NOR serum in protozoa, higher plant and vertebrate cells. J. Cell Sci. 103: 1053-1063 [Abstract]  
  • Colbert, T, Hahn, S (1992). A yeast TFIIB-related factor involved in RNA polymerase III transcription.. Genes Dev. 6: 1940-1949 [Abstract]  
  • Lobo, S M, Lister, J, Sullivan, M L, Hernandez, N (1991). The cloned RNA polymerase II transcription factor IID selects RNA polymerase III to transcribe the human U6 gene in vitro.. Genes Dev. 5: 1477-1489 [Abstract]  
  • Parisi, M., Clayton, D. (1991). Similarity of human mitochondrial transcription factor 1 to high mobility group proteins. Science 252: 965-969 [Abstract]  
  • Bell, S P, Jantzen, H M, Tjian, R (1990). Assembly of alternative multiprotein complexes directs rRNA promoter selectivity.. Genes Dev. 4: 943-954 [Abstract]  
  • Fisher, R P, Parisi, M A, Clayton, D A (1989). Flexible recognition of rapidly evolving promoter sequences by mitochondrial transcription factor 1.. Genes Dev. 3: 2202-2217 [Abstract]  
  • Bell, S., Learned, R., Jantzen, H., Tjian, R (1988). Functional cooperativity between transcription factors UBF1 and SL1 mediates human ribosomal RNA synthesis. Science 241: 1192-1197 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»