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Mol Cell Biol. 1985 September; 5(9): 2221-2230

Lens-specific promoter activity of a mouse gamma-crystallin gene.

S Lok, M L Breitman, A B Chepelinsky, J Piatigorsky, R J Gold and L C Tsui

ABSTRACT

Crystallins are the major water-soluble proteins in vertebrate eye lenses. These lens-specific proteins are encoded by several gene families, and their expression is differentially regulated during lens cell differentiation. Here we show that a cloned mouse gamma-crystallin promoter is active in lens explants derived from 14-day-old chicken embryos but inactive in a variety of cells of non-lens origin. We also show that sequences required for proper utilization of this promoter are contained between nucleotide positions -392 and +47 relative to the transcription initiation site; deletion of sequences from positions -392 to -171 completely abolishes promoter activity. Since chickens do not have gamma-crystallin genes, the expression of a mouse gamma-crystallin promoter in chicken lens cells suggests that different classes of crystallin genes may be regulated by common lens tissue-specific mechanism(s) independent of species.

Mol Cell Biol. 1985 September; 5(9): 2221-2230




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