This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lewis, E D Articles by Manley, J L Search for Related Content PubMed PubMed Citation Articles by Lewis, E D Articles by Manley, J L

 Previous Article  |  Next Article 

Mol Cell Biol. 1985 September; 5(9): 2433-2442

Control of adenovirus late promoter expression in two human cell lines.

ABSTRACT

We investigated the nucleotide sequence requirements of the adenovirus 2 late promoter when activated by either a trans-acting regulatory protein or a cis-acting enhancer element. Using deletion mutants in transient expression assays, we determined that the 5' limit of the region required for activation by a trans-acting regulatory protein, the adenovirus early region 1a gene product, and the simian virus 40 enhancer is the same in both 293 and HeLa cells. Surprisingly, the 3' limit of required sequences varied, depending on the mechanism of activation. Activation mediated by the early region 1a protein endogenous in 293 cells or produced after cotransfection of HeLa cells requires the region around the transcriptional start site, whereas activation brought about by an enhancer element in HeLa cells has no requirement for these sequences. Under no conditions tested did the simian virus 40 enhancer activate the late promoter in 293 cells, even when sequences sufficient for enhancer-mediated activation in HeLa cells, but not for early region 1a activation, were present. These results suggest the existence of at least two different mechanisms for positive regulation of promoter activity.

This article has been cited by other articles:

• Lee, D.-H., Gershenzon, N., Gupta, M., Ioshikhes, I. P., Reinberg, D., Lewis, B. A. (2005). Functional Characterization of Core Promoter Elements: the Downstream Core Element Is Recognized by TAF1. Mol. Cell. Biol. 25: 9674-9686 [Abstract] [Full Text]  
• Kiela, P. R., LeSueur, J., Collins, J. F., Ghishan, F. K. (2003). Transcriptional Regulation of the Rat NHE3 Gene. FUNCTIONAL INTERACTIONS BETWEEN GATA-5 AND Sp FAMILY TRANSCRIPTION FACTORS. J. Biol. Chem. 278: 5659-5668 [Abstract] [Full Text]  
• Emami, K. H., Jain, A., Smale, S. T. (1997). Mechanism of synergy between TATA and initiator: synergistic binding of TFIID following a putative TFIIA-induced isomerization. Genes Dev. 11: 3007-3019 [Abstract] [Full Text]  
• Burke, T. W., Kadonaga, J. T. (1997). The downstream core promoter element, DPE, is conserved from Drosophila to humans and is recognized by TAFII60 of Drosophila. Genes Dev. 11: 3020-3031 [Abstract] [Full Text]  
• Connelly, S, Manley, J L (1988). A functional mRNA polyadenylation signal is required for transcription termination by RNA polymerase II.. Genes Dev. 2: 440-452 [Abstract]  
• Grass, D S, Read, D, Lewis, E D, Manley, J L (1987). Cell- and promoter-specific activation of transcription by DNA replication.. Genes Dev. 1: 1065-1074 [Abstract]  
• Spangler, R, Bruner, M, Dalie, B, Harter, M. (1987). Activation of adenovirus promoters by the adenovirus E1A protein in cell-free extracts. Science 237: 1044-1046 [Abstract]  
• Wright, C., Felber, B., Paskalis, H, Pavlakis, G. (1986). Expression and characterization of the trans-activator of HTLV-III/LAV virus. Science 234: 988-992 [Abstract]  
• Coen, D., Weinheimer, S., McKnight, S. (1986). A genetic approach to promoter recognition during trans induction of viral gene expression. Science 234: 53-59 [Abstract]  
• Jones, K., Kadonaga, J., Luciw, P., Tjian, R (1986). Activation of the AIDS retrovirus promoter by the cellular transcription factor, Sp1. Science 232: 755-759 [Abstract]  
• Lewis, B. A., Kim, T.-K., Orkin, S. H. (2000). A downstream element in the human beta -globin promoter: Evidence of extended sequence-specific transcription factor IID contacts. Proc. Natl. Acad. Sci. USA 97: 7172-7177 [Abstract] [Full Text]